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PMID: 10747028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Histone H2A is required for normal centromere function in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 19 ·No. 7 ·2000-04-03 ·Pages 1598-612

Pinto I, Winston F

Abstract

Histones are structural and functional components of the eukaryotic chromosome, and their function is essential for normal cell cycle progression. In this work, we describe the characterization of two Saccharomyces cerevisiae cold-sensitive histone H2A mutants. Both mutants contain single amino acid replacements of residues predicted to be on the surface of the nucleosome and in close contact with DNA. We show that these H2A mutations cause an increase-in-ploidy phenotype, an increased rate of chromosome loss, and a defect in traversing the G(2)-M phase of the cell cycle. Moreover, these H2A mutations show genetic interactions with mutations in genes encoding kinetochore components. Finally, chromatin analysis of these H2A mutants has revealed an altered centromeric chromatin structure. Taken together, these results strongly suggest that histone H2A is required for proper centromere-kinetochore function during chromosome segregation.

MeSH Terms
Base Sequence Cell Cycle Centromere/metabolism Chromatin/metabolism Cold Temperature DNA Primers/genetics Genes, Fungal Histones/chemistry,genetics,metabolism Kinetochores/metabolism Microtubules/metabolism Models, Molecular Mutagenesis, Site-Directed Nucleosomes/metabolism Phenotype Ploidies Protein Conformation Saccharomyces cerevisiae/cytology,genetics,metabolism Spindle Apparatus/metabolism
Chemicals
Chromatin DNA Primers Histones Nucleosomes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinto I
Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Winston F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.7.1598
Published
2000-04-03
Pages
1598-612
Language
English
Region
England
NLM ID
8208664
PMCID
PMC310229
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032967 · United States
NIGMS NIH HHS · R37 GM032967 · United States
NIGMS NIH HHS · GM32967 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.7.1598 PMC Full Text View journal details
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