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PMID: 8293973 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.

Genetics ·Vol. 135 ·No. 3 ·1993-11-00 ·Pages 677-91

Chan CS, Botstein D

Abstract

We have developed a colony papillation assay for monitoring the copy number of genetically marked chromosomes II and III in Saccharomyces cerevisiae. The unique feature of this assay is that it allows detection of a gain of the marked chromosomes even if there is a gain of the entire set of chromosomes (increase-in-ploidy). This assay was used to screen for chromosome-gain or increase-in-ploidy mutants. Five complementation groups have been defined for recessive mutations that confer an increase-in-ploidy (ipl) phenotype, which, in each case, cosegregates with a temperature-sensitive growth phenotype. Four new alleles of CDC31, which is required for spindle pole body duplication, were also recovered from this screen. Temperature-shift experiments with ipl1 cells show that they suffer severe nondisjunction at 37 degrees. Similar experiments with ipl2 cells show that they gain entire sets of chromosomes and become arrested as unbudded cells at 37 degrees. Molecular cloning and genetic mapping show that IPL1 is a newly identified gene, whereas IPL2 is allelic to BEM2, which is required for normal bud growth.

Related Genes
BEM2 CDC31 ILP2 IPL1
MeSH Terms
Alleles Chromosome Mapping Chromosomes, Fungal Cloning, Molecular DNA, Fungal/metabolism Flow Cytometry Genes, Fungal Genes, Recessive Genetic Complementation Test Genetic Markers Mutation Ploidies Saccharomyces cerevisiae/genetics,metabolism,ultrastructure
Chemicals
DNA, Fungal Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chan C S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Botstein D
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.3.677
Published
1993-11-00
Pages
677-91
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205712
Subset
IM
Grants
NIGMS NIH HHS · GM45185 · United States
NIGMS NIH HHS · GM46406 · United States
External Links
PubMed source DOI: 10.1093/genetics/135.3.677 PMC Full Text View journal details
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