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PMID: 10756204 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Functional characterization of Caenorhabditis elegans DNA topoisomerase IIIalpha.

Nucleic acids research ·Vol. 28 ·No. 9 ·2000-05-01 ·Pages 2012-7

Kim YC, Lee J, Koo HS

Abstract

To investigate the function of a DNA topoisomerase III enzyme in Caenorhabditis elegans, the full-length cDNA of C.elegans DNA topoisomerase IIIalpha was cloned. The deduced amino acid sequence exhibited identities of 48 and 39% with those of human DNA topoisomerase IIIalpha and Saccharomyces cerevisiae DNA topoisomerase III, respectively. The overexpressed polypeptide showed an optimal activity for removing negative DNA supercoils at a relatively high temperature of 52-57 degrees C, which is similar to the optimum temperatures of other eukaryotic DNA topoisomerase III enzymes. When topoisomerase IIIalpha expression was interfered with by a cognate double-stranded RNA injection, pleiotropic phenotypes with abnormalities in germ cell proliferation, oogenesis and embryo-genesis appeared. These phenotypes were well correlated with mRNA expression localized in the meiotic cells of gonad and early embryonic cells.

MeSH Terms
Animals Caenorhabditis elegans/embryology,enzymology,genetics Catalysis Cloning, Molecular DNA Topoisomerases, Type I/genetics,metabolism DNA, Complementary/chemistry,genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic/drug effects Genes/genetics Glutathione Transferase/genetics,metabolism In Situ Hybridization Molecular Sequence Data RNA, Antisense/administration & dosage,genetics RNA, Messenger/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Sequence Analysis, DNA Topoisomerase I Inhibitors
Chemicals
DNA, Complementary RNA, Antisense RNA, Messenger Recombinant Fusion Proteins Topoisomerase I Inhibitors Glutathione Transferase DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim Y C
Department of Biochemistry, College of Science, Yonsei University, Seoul, Korea.
Lee J
Koo H S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/28.9.2012
Published
2000-05-01
Pages
2012-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC103277
Subset
IM
Databases
GENBANK
AF057032
External Links
PubMed source DOI: 10.1093/nar/28.9.2012 PMC Full Text View journal details
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