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PMID: 7969174 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase.

Molecular and cellular biology ·Vol. 14 ·No. 12 ·1994-12-00 ·Pages 8391-8

Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R

Abstract

We have previously shown that cells mutant for TOP3, a gene encoding a prokaryotic-like type I topoisomerase in Saccharomyces cerevisiae, display a pleiotropic phenotype including slow growth and genome instability. We identified a mutation, sgs1 (slow growth suppressor), that suppresses both the growth defect and the increased genomic instability of top3 mutants. Here we report the independent isolation of the SGS1 gene in a screen for proteins that interact with Top3. DNA sequence analysis reveals that the putative Sgs1 protein is highly homologous to the helicase encoded by the Escherichia coli recQ gene. These results imply that Sgs1 creates a deleterious topological substrate that Top3 preferentially resolves. The interaction of the Sgs1 helicase homolog and the Top3 topoisomerase is reminiscent of the recently described structure of reverse gyrase from Sulfolobus acidocaldarius, in which a type I DNA topoisomerase and a helicase-like domain are fused in a single polypeptide.

Related Genes
SGS1 TOP3 recQ
MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Cloning, Molecular DNA Helicases/chemistry DNA Primers/chemistry DNA Topoisomerases, Type I/metabolism Fungal Proteins/genetics,metabolism Genes, Fungal Genes, Suppressor Molecular Sequence Data Protein Binding RecQ Helicases Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid
Chemicals
DNA Primers Fungal Proteins Saccharomyces cerevisiae Proteins SGS1 protein, S cerevisiae DNA Helicases RecQ Helicases DNA Topoisomerases, Type I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gangloff S
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
McDonald J P
Bendixen C
Arthur L
Rothstein R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.12.8391-8398.1994
Published
1994-12-00
Pages
8391-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359378
Subset
IM
Grants
NCI NIH HHS · CA21111 · United States
NIGMS NIH HHS · GM34587 · United States
NHGRI NIH HHS · HG00452 · United States
Databases
GENBANK
D37984, U00052
External Links
PubMed source DOI: 10.1128/mcb.14.12.8391-8398.1994 PMC Full Text View journal details
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