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PMID: 10758151 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 9 ·2000-04-25 ·Pages 4885-90

Rashid MH, Kornberg A

Abstract

Polyphosphate kinase (PPK), encoded by the ppk gene, is the principal enzyme in many bacteria for the synthesis of inorganic polyphosphate (poly P) from ATP. A knockout mutant in the ppk gene of Pseudomonas aeruginosa PAO1 is impaired in flagellar swimming motility on semisolid agar plates. The mutant is deficient in type IV pili-mediated twitching motility and in a "swarming motility" previously unobserved in P. aeruginosa. In swarming cultures, the polar monotrichous bacteria have differentiated into elongated and polar multitrichous cells that navigate the surface of solid media. All of the motility defects in the ppk mutant could be complemented by a plasmid harboring the ppk gene. Because bacterial motility is often crucial for their survival in a natural environment and for systemic infection inside a host, the dependence for motility on PPK reveals important roles for poly P in diverse processes such as biofilm formation, symbiosis, and virulence.

MeSH Terms
Agar Fimbriae, Bacterial/physiology Flagella/physiology Gene Deletion Genes, Bacterial Movement/physiology Phosphotransferases (Phosphate Group Acceptor)/genetics,metabolism Polyphosphates/metabolism Pseudomonas aeruginosa/cytology,genetics,physiology
Chemicals
Polyphosphates Agar Phosphotransferases (Phosphate Group Acceptor) polyphosphate kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rashid M H
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.
Kornberg A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.060030097
Published
2000-04-25
Pages
4885-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18327
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.060030097 PMC Full Text View journal details
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