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PMID: 10769039 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plakoglobin suppresses epithelial proliferation and hair growth in vivo.

The Journal of cell biology ·Vol. 149 ·No. 2 ·2000-04-17 ·Pages 503-20

Charpentier E, Lavker RM, Acquista E, Cowin P

Abstract

Plakoglobin regulates cell adhesion by providing a modulatable connection between both classical and desmosomal cadherins and their respective cytoskeletal linker proteins. Both plakoglobin and the related protein beta-catenin are posttranscriptionally upregulated in response to Wnt-1 in cultured cells. Upregulation of beta-catenin has been implicated in potentiating hyperproliferation and tumor formation. To investigate the role of plakoglobin in these functions we expressed a full-length (PG) and an NH(2)-terminally truncated form of plakoglobin (DeltaN80PG) in mouse epidermis and hair follicles, tissues which undergo continuous and easily observed postnatal renewal and remodeling. Expression of these constructs results in stunted hair growth, a phenotype that has also been observed in transgenic mice expressing Wnt3 and Dvl2 (Millar et al. 1999). Hair follicles from PG and DeltaN80PG mice show premature termination of the growth phase (anagen) of the hair cycle, an event that is regulated in part by FGF5 (Hebert et al. 1994). The proliferative rate of the epidermal cells was reduced and apoptotic changes, which are associated with entry into the regressive phase of the hair follicle cycle (catagen), occurred earlier than usual.

MeSH Terms
Aging Animals Base Sequence Cell Adhesion Molecules/genetics,physiology Cell Division/physiology Cell Line, Transformed Cytoskeletal Proteins/genetics,physiology Desmoplakins Desmosomes/physiology,ultrastructure Epidermal Cells Epidermis/ultrastructure Epithelial Cells/cytology,ultrastructure Hair Follicle/cytology,growth & development,ultrastructure Humans Mice Mice, Transgenic Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Recombinant Proteins/metabolism Sequence Deletion Transfection gamma Catenin
Chemicals
Cell Adhesion Molecules Cytoskeletal Proteins Desmoplakins Oligodeoxyribonucleotides Recombinant Proteins gamma Catenin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charpentier E
Departments of Cell Biology and Dermatology, New York University Medical School, New York 10016, USA.
Lavker R M
Acquista E
Cowin P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.2.503
Published
2000-04-17
Pages
503-20
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175163
Subset
IM
Grants
NIGMS NIH HHS · GM47429 · United States
External Links
PubMed source DOI: 10.1083/jcb.149.2.503 PMC Full Text View journal details
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