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PMID: 8408227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.

The Journal of cell biology ·Vol. 123 ·No. 2 ·1993-10-00 ·Pages 477-84

McCrea PD, Brieher WM, Gumbiner BM

Abstract

We have obtained evidence that a known intracellular component of the cadherin cell-cell adhesion machinery, beta-catenin, contributes to the development of the body axis in the frog Xenopus laevis. Vertebrate beta-catenin is homologous to the Drosophila segment polarity gene product armadillo, and to vertebrate plakoglobin (McCrea, P. D., C. W. Turck, and B. Gumbiner. 1991. Science (Wash. DC). 254: 1359-1361.). Beta-Catenin was found present in all Xenopus embryonic stages examined, and associated with C-cadherin, the major cadherin present in early Xenopus embryos. To test beta-catenin's function, affinity purified Fab fragments were injected into ventral blastomeres of developing four-cell Xenopus embryos. A dramatic phenotype, the duplication of the dorsoanterior embryonic axis, was observed. Furthermore, Fab injections were capable of rescuing dorsal features in UV-ventralized embryos. Similar phenotypes have been observed in misexpression studies of the Wnt and other gene products, suggesting that beta-catenin participates in a signaling pathway which specifies embryonic patterning.

MeSH Terms
Animals Antibody Specificity Cadherins/analysis Cell Adhesion/drug effects,physiology Cell Division/drug effects,physiology Cytoskeletal Proteins/analysis,immunology,physiology Embryo, Mammalian/chemistry,physiology Embryo, Nonmammalian Embryonic and Fetal Development/drug effects,physiology Female Gene Expression Growth Substances/genetics,physiology Humans Immunoglobulin Fab Fragments/administration & dosage,immunology,pharmacology Microinjections Phenotype Trans-Activators Xenopus Proteins Xenopus laevis/embryology,physiology beta Catenin
Chemicals
CTNNB1 protein, Xenopus CTNNB1 protein, human Cadherins Cytoskeletal Proteins Growth Substances Immunoglobulin Fab Fragments Trans-Activators Xenopus Proteins beta Catenin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCrea P D
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
Brieher W M
Gumbiner B M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.123.2.477
Published
1993-10-00
Pages
477-84
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119835
Subset
IM
Grants
NIGMS NIH HHS · GM37432 · United States
NCI NIH HHS · NCI-P30-CA-08748 · United States
External Links
PubMed source DOI: 10.1083/jcb.123.2.477 PMC Full Text View journal details
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