Home LiteratureArticle Details
PMID: 10790369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The tight junction protein ZO-1 and an interacting transcription factor regulate ErbB-2 expression.

The EMBO journal ·Vol. 19 ·No. 9 ·2000-05-02 ·Pages 2024-33

Balda MS, Matter K

Abstract

Epithelial tight junctions regulate paracellular diffusion and restrict the intermixing of apical and basolateral plasma membrane components. We now identify a Y-box transcription factor, ZONAB (ZO-1-associated nucleic acid-binding protein), that binds to the SH3 domain of ZO-1, a submembrane protein of tight junctions. ZONAB localizes to the nucleus and at tight junctions, and binds to sequences of specific promoters containing an inverted CCAAT box. In reporter assays, ZONAB and ZO-1 functionally interact in the regulation of the ErbB-2 promoter in a cell density-dependent manner. In stably transfected overexpressing cells, ZO-1 and ZONAB control expression of endogenous ErbB-2 and function in the regulation of paracellular permeability. These data indicate that tight junctions directly participate in the control of gene expression and suggest that they function in the regulation of epithelial cell differentiation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Count Cell Line Cell Membrane Permeability Cell Nucleus/chemistry Cloning, Molecular DNA/genetics,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Dogs Gene Expression Regulation Genes, cdc Genes, erbB-2/genetics Membrane Proteins/chemistry,genetics,metabolism Molecular Sequence Data Mutation/genetics Phosphoproteins/chemistry,genetics,metabolism Precipitin Tests Promoter Regions, Genetic/genetics Protein Binding Tight Junctions/chemistry Transcription Factors/chemistry,genetics,metabolism Transfection Zonula Occludens-1 Protein src Homology Domains
Chemicals
DNA-Binding Proteins Membrane Proteins Phosphoproteins Transcription Factors ZONAB protein, Canis familiaris Zonula Occludens-1 Protein DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Balda M S
Department of Cell Biology, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Genève-4, Switzerland. [email protected]
Matter K
References (43)
43 references, click to expand
  1. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
    J Cell Biol. 1986 Sep;103(3):755-66 PMID: 3528172
  2. Junctional complexes in various epithelia.
    J Cell Biol. 1963 May;17:375-412 PMID: 13944428
  3. Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology.
    Gene. 1988 Dec 20;73(2):499-507 PMID: 2977358
  4. The 68 kDa protein of signal recognition particle contains a glycine-rich region also found in certain RNA-binding proteins.
    FEBS Lett. 1990 Dec 10;276(1-2):103-7 PMID: 1702390
  5. Basolateral sorting of LDL receptor in MDCK cells: the cytoplasmic domain contains two tyrosine-dependent targeting determinants.
    Cell. 1992 Nov 27;71(5):741-53 PMID: 1423629
  6. Two classes of tight junctions are revealed by ZO-1 isoforms.
    Am J Physiol. 1993 Apr;264(4 Pt 1):C918-24 PMID: 7682777
  7. The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions.
    Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7834-8 PMID: 8395056
  8. Submembranous junctional plaque proteins include potential tumor suppressor molecules.
    J Cell Biol. 1993 Dec;123(5):1049-53 PMID: 8245116
  9. Association of the APC gene product with beta-catenin.
    Science. 1993 Dec 10;262(5140):1731-4 PMID: 8259518
  10. Association of the APC tumor suppressor protein with catenins.
    Science. 1993 Dec 10;262(5140):1734-7 PMID: 8259519
  11. Structural features of the 5' region of the human erbB-2 gene.
    Gene. 1993 Dec 22;136(1-2):361-4 PMID: 7904974
  12. ZO-1, DlgA and PSD-95/SAP90: homologous proteins in tight, septate and synaptic cell junctions.
    Mech Dev. 1993 Dec;44(2-3):85-9 PMID: 8155583
  13. Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.
    J Cell Biol. 1994 Dec;127(6 Pt 1):1617-26 PMID: 7798316
  14. E-cadherin as a tumor (invasion) suppressor gene.
    Bioessays. 1995 Feb;17(2):97-9 PMID: 7748170
  15. Not just glue: cell-cell junctions as cellular signaling centers.
    Curr Opin Genet Dev. 1995 Feb;5(1):56-65 PMID: 7749327
  16. Cell adhesion: the molecular basis of tissue architecture and morphogenesis.
    Cell. 1996 Feb 9;84(3):345-57 PMID: 8608588
  17. Masking of mRNA by Y-box proteins.
    FASEB J. 1996 Mar;10(4):435-43 PMID: 8647342
  18. Catenins and zonula occludens-1 form a complex during early stages in the assembly of tight junctions.
    J Cell Biol. 1996 Feb;132(3):451-63 PMID: 8636221
  19. The Drosophila tamou gene, a component of the activating pathway of extramacrochaetae expression, encodes a protein homologous to mammalian cell-cell junction-associated protein ZO-1.
    Genes Dev. 1996 Jul 15;10(14):1783-95 PMID: 8698238
  20. Functional interaction of beta-catenin with the transcription factor LEF-1.
    Nature. 1996 Aug 15;382(6592):638-42 PMID: 8757136
  21. XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos.
    Cell. 1996 Aug 9;86(3):391-9 PMID: 8756721
  22. Symplekin, a novel type of tight junction plaque protein.
    J Cell Biol. 1996 Aug;134(4):1003-18 PMID: 8769423
  23. Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein.
    J Cell Biol. 1996 Aug;134(4):1031-49 PMID: 8769425
  24. The junction-associated protein, zonula occludens-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts.
    Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10779-84 PMID: 8855257
  25. Nuclear localization of beta-catenin by interaction with transcription factor LEF-1.
    Mech Dev. 1996 Sep;59(1):3-10 PMID: 8892228
  26. The SH3 domain of the tight junction protein ZO-1 binds to a serine protein kinase that phosphorylates a region C-terminal to this domain.
    FEBS Lett. 1996 Dec 16;399(3):326-32 PMID: 8985173
  27. Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments.
    J Cell Biol. 1997 Jul 14;138(1):181-92 PMID: 9214391
  28. The ErbB signaling network in embryogenesis and oncogenesis: signal diversification through combinatorial ligand-receptor interactions.
    FEBS Lett. 1997 Jun 23;410(1):83-6 PMID: 9247128
  29. Cadherins, catenins and APC protein: interplay between cytoskeletal complexes and signaling pathways.
    Curr Opin Cell Biol. 1997 Oct;9(5):683-90 PMID: 9330872
  30. Induction of epithelial branching tubulogenesis in vitro.
    J Cell Physiol. 1997 Nov;173(2):152-61 PMID: 9365515
  31. Organizing a functional junctional complex requires specific domains of the Drosophila MAGUK Discs large.
    Genes Dev. 1997 Dec 1;11(23):3242-53 PMID: 9389655
  32. Tight junctions.
    J Cell Sci. 1998 Mar;111 ( Pt 5):541-7 PMID: 9454728
  33. Direct involvement of the Y-box binding protein YB-1 in genotoxic stress-induced activation of the human multidrug resistance 1 gene.
    J Biol Chem. 1998 Mar 13;273(11):5997-6000 PMID: 9497311
  34. Regulation of the movement of solutes across tight junctions.
    Annu Rev Physiol. 1998;60:143-59 PMID: 9558458
  35. Integrin signaling and cell growth control.
    Curr Opin Cell Biol. 1998 Apr;10(2):220-31 PMID: 9561846
  36. TCF: transcriptional activator or repressor?
    Curr Opin Cell Biol. 1998 Jun;10(3):366-72 PMID: 9640538
  37. Gene regulation by Y-box proteins: coupling control of transcription and translation.
    Trends Cell Biol. 1998 Aug;8(8):318-23 PMID: 9704408
  38. Occludin and the functions of tight junctions.
    Int Rev Cytol. 1999;186:117-46 PMID: 9770298
  39. Reconstitution of mammary gland development in vitro: requirement of c-met and c-erbB2 signaling for branching and alveolar morphogenesis.
    J Cell Biol. 1998 Oct 19;143(2):533-45 PMID: 9786961
  40. The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton.
    J Biol Chem. 1998 Nov 6;273(45):29745-53 PMID: 9792688
  41. Loss of the tight junction MAGUK ZO-1 in breast cancer: relationship to glandular differentiation and loss of heterozygosity.
    Am J Pathol. 1998 Dec;153(6):1767-73 PMID: 9846967
  42. Structural and signalling molecules come together at tight junctions.
    Curr Opin Cell Biol. 1999 Oct;11(5):628-33 PMID: 10508648
  43. Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells.
    J Cell Biol. 1988 Apr;106(4):1141-9 PMID: 2452168
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.9.2024
Email
[email protected]
Published
2000-05-02
Pages
2024-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305688
Subset
IM
Databases
GENBANK
AF171061, AF171062
External Links
PubMed source DOI: 10.1093/emboj/19.9.2024 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2