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PMID: 3528172 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.

The Journal of cell biology ·Vol. 103 ·No. 3 ·1986-09-00 ·Pages 755-66

Stevenson BR, Siliciano JD, Mooseker MS, Goodenough DA

Abstract

A tight junction-enriched membrane fraction has been used as immunogen to generate a monoclonal antiserum specific for this intercellular junction. Hybridomas were screened for their ability to both react on an immunoblot and localize to the junctional complex region on frozen sections of unfixed mouse liver. A stable hybridoma line has been isolated that secretes an antibody (R26.4C) that localizes in thin section images of isolated mouse liver plasma membranes to the points of membrane contact at the tight junction. This antibody recognizes a polypeptide of approximately 225,000 D, detectable in whole liver homogenates as well as in the tight junction-enriched membrane fraction. R26.4C localizes to the junctional complex region of a number of other epithelia, including colon, kidney, and testis, and to arterial endothelium, as assayed by immunofluorescent staining of cryostat sections of whole tissue. This antibody also stains the junctional complex region in confluent monolayers of the Madin-Darby canine kidney epithelial cell line. Immunoblot analysis of Madin-Darby canine kidney cells demonstrates the presence of a polypeptide similar in molecular weight to that detected in liver, suggesting that this protein is potentially a ubiquitous component of all mammalian tight junctions. The 225-kD tight junction-associated polypeptide is termed "ZO-1."

MeSH Terms
Animals Antibody Specificity Dogs Epithelium/analysis,ultrastructure Fluorescent Antibody Technique Intercellular Junctions/analysis Mice Peptides/immunology,isolation & purification Rats
Chemicals
Peptides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stevenson B R
Siliciano J D
Mooseker M S
Goodenough D A
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39 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.3.755
Published
1986-09-00
Pages
755-66
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114282
Subset
IM
Grants
NIGMS NIH HHS · GM-28932 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.3.755 PMC Full Text View journal details
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