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PMID: 10791971 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis.

The Journal of cell biology ·Vol. 149 ·No. 3 ·2000-05-01 ·Pages 567-74

Ko YG, Kang YS, Kim EK, Park SG, Kim S

Abstract

Human aminoacyl-tRNA synthetases (ARSs) are normally located in cytoplasm and are involved in protein synthesis. In the present work, we found that human methionyl-tRNA synthetase (MRS) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of MRS was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of MRS in nucleoli depended on the integrity of RNA and the activity of RNA polymerase I in the nucleolus. The ribosomal RNA synthesis was specifically decreased by the treatment of anti-MRS antibody as determined by nuclear run-on assay and immunostaining with anti-Br antibody after incorporating Br-UTP into nascent RNA. Thus, human MRS plays a role in the biogenesis of rRNA in nucleoli, while it is catalytically involved in protein synthesis in cytoplasm.

MeSH Terms
Antibodies/pharmacology Biological Transport/drug effects Cell Division Cell Nucleolus/enzymology Cells, Cultured Deoxyuracil Nucleotides/immunology,metabolism Epidermal Growth Factor/pharmacology Fluorescent Antibody Technique Humans Immunoblotting Insulin/pharmacology Methionine-tRNA Ligase/metabolism Nuclear Proteins/chemistry Platelet-Derived Growth Factor/pharmacology RNA Polymerase I/metabolism RNA, Nuclear/chemistry RNA, Ribosomal/biosynthesis
Chemicals
Antibodies Deoxyuracil Nucleotides Insulin Nuclear Proteins Platelet-Derived Growth Factor RNA, Nuclear RNA, Ribosomal bromodeoxyuridine triphosphate Epidermal Growth Factor RNA Polymerase I Methionine-tRNA Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ko Y G
National Creative Research Initiatives Center for ARS Network, Sung Kyun Kwan University, Jangangu, Suwon, Kyunggido 440-746, Korea.
Kang Y S
Kim E K
Park S G
Kim S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.3.567
Published
2000-05-01
Pages
567-74
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2174846
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.149.3.567 PMC Full Text View journal details
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