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PMID: 8895587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases.

The EMBO journal ·Vol. 15 ·No. 19 ·1996-10-01 ·Pages 5437-48

Simos G, Segref A, Fasiolo F, Hellmuth K, Shevchenko A, Mann M, Hurt EC

Abstract

Arc1p was found in a screen for components that interact genetically with Los1p, a nuclear pore-associated yeast protein involved in tRNA biogenesis. Arc1p is associated with two proteins which were identified as methionyl-tRNA and glutamyl-tRNA synthetase (MetRS and GluRS) by a new mass spectrometry method. ARC1 gene disruption leads to slow growth and reduced MetRS activity, and synthetically lethal arc1- mutants are complemented by the genes for MetRS and GluRS. Recombinant Arc1p binds in vitro to purified monomeric yeast MetRS, but not to an N-terminal truncated form, and strongly increases its apparent affinity for tRNAMet. Furthermore, Arc1p, which is allelic to the quadruplex nucleic acid binding protein G4p1, exhibits specific binding to tRNA as determined by gel retardation and UV-cross-linking. Arc1p is, therefore, a yeast protein with dual specificity: it associates with tRNA and aminoacyl-tRNA synthetases. This functional interaction may be required for efficient aminoacylation in vivo.

MeSH Terms
Acylation Amino Acid Sequence Cytoplasm/chemistry Genes, Fungal/genetics Glutamate-tRNA Ligase/metabolism Kinetics Membrane Proteins/metabolism Methionine-tRNA Ligase/metabolism Molecular Sequence Data Mutation Nuclear Envelope/metabolism RNA, Transfer/metabolism RNA-Binding Proteins/analysis,genetics,metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Yeasts/enzymology,genetics,growth & development
Chemicals
ARC1 protein, S cerevisiae Membrane Proteins RNA-Binding Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins RNA, Transfer Methionine-tRNA Ligase Glutamate-tRNA Ligase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Simos G
University of Heidelberg, Institut für Biochemie I, Germany.
Segref A
Fasiolo F
Hellmuth K
Shevchenko A
Mann M
Hurt E C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-10-01
Pages
5437-48
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452286
Subset
IM
Databases
GENBANK
X95481
External Links
PubMed source PMC Full Text View journal details
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