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PMID: 7509815 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear export of different classes of RNA is mediated by specific factors.

The Journal of cell biology ·Vol. 124 ·No. 5 ·1994-03-00 ·Pages 627-35

Jarmolowski A, Boelens WC, Izaurralde E, Mattaj IW

Abstract

Various classes of RNA are exported from the nucleus to the cytoplasm, including transcripts of RNA polymerase I (large ribosomal RNAs), II (U-rich small nuclear RNAs [U snRNAs], mRNAs), and III (tRNAs, 5S RNA). Here, evidence is presented that some steps in the export of various classes of nuclear RNA are mediated by specific rather than common factors. Using microinjection into Xenopus oocytes, it is shown that a tRNA, a U snRNA, and an mRNA competitively inhibit their own export at concentrations at which they have no effect on the export of heterologous RNAs. While the export of both U snRNAs and mRNAs is enhanced by their 7-methyl guanosine cap structures, factors recognizing this structure are found to be limiting in concentration only in the case of U snRNAs. In addition to the specific factors, evidence for steps in the export process that may be common to at least some classes of RNA are provided by experiments in which synthetic homopolymeric RNAs are used as inhibitors.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism DNA Primers Female Humans Kinetics Microinjections Molecular Sequence Data Oocytes/metabolism Polymerase Chain Reaction RNA/administration & dosage,biosynthesis,metabolism RNA Polymerase I/metabolism RNA Polymerase II/metabolism RNA, Messenger/metabolism RNA, Small Nuclear/metabolism RNA, Transfer, Met/biosynthesis,metabolism Xenopus laevis
Chemicals
DNA Primers RNA, Messenger RNA, Small Nuclear RNA, Transfer, Met RNA RNA Polymerase II RNA Polymerase I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jarmolowski A
European Molecular Biology Laboratory, Heidelberg, Germany.
Boelens W C
Izaurralde E
Mattaj I W
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49 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.124.5.627
Published
1994-03-00
Pages
627-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119953
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.124.5.627 PMC Full Text View journal details
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