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PMID: 2459127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

The Journal of cell biology ·Vol. 107 ·No. 4 ·1988-10-00 ·Pages 1289-97

Featherstone C, Darby MK, Gerace L

Abstract

A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react with similar components in Xenopus oocytes, as determined by immunofluorescence microscopy and immunoblotting. We have microinjected the antibody into oocytes to study the possible role of these polypeptides in nucleocytoplasmic transport. The antibody inhibits import of a large nuclear protein, nucleoplasmin, in a time- and concentration-dependent manner. It also inhibits export of 5S ribosomal RNA and mature tRNA, but has no effect on transcription or intranuclear tRNA processing. The antibody does not affect the rate of diffusion into the nucleus of two small proteins, myoglobin and ovalbumin, indicating that antibody binding does not result in occlusion of the channel for diffusion. This suggests that inhibition of protein and RNA transport occurs by binding of the antibody at or near components of the pore that participate in mediated transport.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Biological Transport Blotting, Western Diffusion Fluorescent Antibody Technique Immunologic Techniques Nuclear Envelope/immunology,physiology Oocytes Proteins/metabolism RNA/metabolism Xenopus laevis
Chemicals
Antibodies, Monoclonal Proteins RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Featherstone C
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Darby M K
Gerace L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.4.1289
Published
1988-10-00
Pages
1289-97
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115252
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.107.4.1289 PMC Full Text View journal details
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