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PMID: 1531146 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.

The Journal of cell biology ·Vol. 116 ·No. 4 ·1992-02-00 ·Pages 851-61

Michaud N, Goldfarb D

Abstract

The inhibitory effects of wheat germ agglutinin and mAb 414 on the nuclear import of all types of U snRNAs indicate that they cross the nuclear envelope through the nuclear pore complex. However, the import of different U snRNAs occurs by kinetically distinct targeting pathways that can be distinguished from one another by the competitive effects of free trimethylguanosine cap dinucleotide (m3GpppG) and P(Lys)-BSA, an efficient synthetic karyophile based on the nuclear localization signal of SV40 large T antigen. The import of U snRNAs that contain 5' m3GpppN caps and are complexed by Sm proteins (U1, U2, U4, and U5) is competed by coinjection with free m3GpppG, indicating a shared transport factor, but not by P(Lys)-BSA. The import of U6 snRNA, which lacks a m3GpppN cap and is not complexed by the Sm proteins, is competed by P(Lys)-BSA but not by free m3GpppG. Thus, by the criterion of kinetic competition, U6 snRNA import is identical to that of the karyophilic proteins P(Lys)-BSA and nucleoplasmin. Uniquely, the import of U3 snRNA, which contains a m3GpppN cap but does not bind Sm proteins is not competed by either free m3GpppG or P(Lys)-BSA. Thus, U3 snRNA appears to be imported by a novel third kinetic pathway.

MeSH Terms
Amino Acid Sequence Animals Cell Nucleus/metabolism HeLa Cells Humans Microinjections Molecular Sequence Data Nuclear Envelope/metabolism Oocytes/metabolism RNA Cap Analogs/pharmacology RNA, Small Nuclear/metabolism Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Wheat Germ Agglutinins Xenopus
Chemicals
RNA Cap Analogs RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear Wheat Germ Agglutinins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Michaud N
Department of Biology, University of Rochester, New York 14627.
Goldfarb D
References (56)
56 references, click to expand
  1. B2 RNA and 7SK RNA, RNA polymerase III transcripts, have a cap-like structure at their 5' end.
    Nucleic Acids Res. 1990 Nov 11;18(21):6347-51 PMID: 1700854
  2. The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
    Cell. 1990 Aug 10;62(3):569-77 PMID: 2143105
  3. Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
    EMBO J. 1990 Oct;9(10):3397-404 PMID: 2145149
  4. A nuclear cap binding protein from HeLa cells.
    Nucleic Acids Res. 1990 Dec 11;18(23):6989-95 PMID: 2148205
  5. Visualization of transport-related configurations of the nuclear pore transporter.
    Biophys J. 1990 Aug;58(2):341-55 PMID: 2207242
  6. Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
    J Cell Biol. 1990 Oct;111(4):1571-82 PMID: 2211825
  7. Monomethylated cap structures facilitate RNA export from the nucleus.
    Cell. 1990 Oct 5;63(1):109-18 PMID: 2208274
  8. An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore.
    J Cell Biol. 1990 Mar;110(3):547-57 PMID: 2307698
  9. Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
    Mol Cell Biol. 1990 Jul;10(7):3365-75 PMID: 2355910
  10. Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
    J Cell Biol. 1990 Sep;111(3):807-16 PMID: 2391365
  11. A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
    J Cell Biol. 1988 Oct;107(4):1289-97 PMID: 2459127
  12. Nuclear transport.
    Curr Opin Cell Biol. 1989 Jun;1(3):441-6 PMID: 2483331
  13. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
    EMBO J. 1989 Oct;8(10):3113-9 PMID: 2531075
  14. Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
    Cell. 1985 Jan;40(1):111-8 PMID: 2578319
  15. Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8280-3 PMID: 2813391
  16. In vitro assembly of U1 snRNPs.
    EMBO J. 1987 Nov;6(11):3479-85 PMID: 2962858
  17. Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
    Science. 1988 Sep 9;241(4871):1328-31 PMID: 2970672
  18. Effect of basic and nonbasic amino acid substitutions on transport induced by simian virus 40 T-antigen synthetic peptide nuclear transport signals.
    Mol Cell Biol. 1988 Jul;8(7):2722-9 PMID: 3043192
  19. Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
    Cell. 1988 Mar 11;52(5):655-64 PMID: 3125984
  20. Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.
    Nucleic Acids Res. 1988 Oct 25;16(20):9415-29 PMID: 3141901
  21. Beta-globin mRNAs capped with m7G, m2.7(2)G or m2.2.7(3)G differ in intrinsic translation efficiency.
    Nucleic Acids Res. 1988 Sep 26;16(18):8953-62 PMID: 3174438
  22. Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin.
    Exp Cell Res. 1988 Jan;174(1):291-6 PMID: 3335228
  23. Identification and characterization of a nuclear pore complex protein.
    Cell. 1986 Jun 6;45(5):699-709 PMID: 3518946
  24. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.
    J Cell Biol. 1987 May;104(5):1157-64 PMID: 3571327
  25. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
    J Cell Biol. 1987 Feb;104(2):189-200 PMID: 3805121
  26. Intracellular transport of microinjected 5S and small nuclear RNAs.
    Nature. 1982 Feb 18;295(5850):572-7 PMID: 6173771
  27. Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.
    EMBO J. 1984 Jun;3(6):1357-63 PMID: 6204860
  28. 5'-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Double-label immunofluorescent studies with anti-m3G antibodies and with anti-RNP and anti-Sm autoantibodies.
    Exp Cell Res. 1984 Oct;154(2):548-60 PMID: 6207038
  29. RNA processing and ribonucleoprotein assembly studied in vivo by RNA transfection.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1283-7 PMID: 2137610
  30. Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
    Cell. 1991 Sep 6;66(5):837-47 PMID: 1653647
  31. Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport.
    J Cell Biol. 1991 May;113(4):705-14 PMID: 1827444
  32. Nuclear protein localization.
    Biochim Biophys Acta. 1991 Mar 7;1071(1):83-101 PMID: 2004116
  33. Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.
    J Cell Biol. 1987 May;104(5):1143-56 PMID: 2437126
  34. A spectroscopic study of the binding of N-7-substituted cap analogues to human protein synthesis initiation factor 4E.
    Biochemistry. 1990 Apr 3;29(13):3337-41 PMID: 2334695
  35. An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
    Science. 1990 Aug 17;249(4970):786-90 PMID: 2143847
  36. Protein import through the nuclear pore complex is a multistep process.
    J Cell Biol. 1989 Sep;109(3):971-82 PMID: 2475512
  37. An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
    EMBO J. 1989 Dec 20;8(13):4179-87 PMID: 2531660
  38. A murine monoclonal antibody recognizes the 13,000 molecular weight polypeptide of the Sm small nuclear ribonucleoprotein complex.
    J Immunol. 1985 Jul;135(1):428-32 PMID: 2582042
  39. Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
    Cell. 1986 Sep 12;46(6):905-11 PMID: 2944599
  40. The effect of protein context on nuclear location signal function.
    Cell. 1987 Jul 31;50(3):465-75 PMID: 3038333
  41. Monoclonal antibodies to a Mr 68,000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes.
    Chromosoma. 1988 Nov;97(3):193-7 PMID: 3064988
  42. The effects of variations in the number and sequence of targeting signals on nuclear uptake.
    J Cell Biol. 1988 Oct;107(4):1279-87 PMID: 3170630
  43. Cap recognition and the entry of mRNA into the protein synthesis initiation cycle.
    Trends Biochem Sci. 1988 Feb;13(2):52-6 PMID: 3238751
  44. Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
    Cell. 1988 Mar 11;52(5):641-53 PMID: 3345567
  45. Synthetic peptides as nuclear localization signals.
    Nature. 1986 Aug 14-20;322(6080):641-4 PMID: 3638500
  46. Sequence requirements for nuclear location of simian virus 40 large-T antigen.
    Nature. 1984 Sep 6-11;311(5981):33-8 PMID: 6088992
  47. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
    Nucleic Acids Res. 1984 Apr 11;12(7):3283-93 PMID: 6201826
  48. Movement of a karyophilic protein through the nuclear pores of oocytes.
    J Cell Biol. 1984 Dec;99(6):2216-22 PMID: 6501421
  49. Facilitated nuclear transport of histone H1 and other small nucleophilic proteins.
    Cell. 1990 Mar 23;60(6):999-1008 PMID: 1690602
  50. An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
    EMBO J. 1991 Sep;10(9):2645-51 PMID: 1714385
  51. Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway.
    J Cell Biol. 1991 Jan;112(2):215-23 PMID: 1824847
  52. Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.
    Cell. 1991 May 3;65(3):517-26 PMID: 1826860
  53. How proteins enter the nucleus.
    Cell. 1991 Feb 8;64(3):489-97 PMID: 1991319
  54. A yeast protein that binds nuclear localization signals: purification localization, and antibody inhibition of binding activity.
    J Cell Biol. 1991 Jun;113(6):1243-54 PMID: 2045410
  55. Comparison of diverse transport signals in synthetic peptide-induced nuclear transport.
    Exp Cell Res. 1990 Jan;186(1):32-8 PMID: 2137089
  56. A new U6 small nuclear ribonucleoprotein-specific protein conserved between cis- and trans-splicing systems.
    Mol Cell Biol. 1991 Apr;11(4):2026-34 PMID: 1826044
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.116.4.851
Published
1992-02-00
Pages
851-61
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289328
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.116.4.851 PMC Full Text View journal details
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