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PMID: 2531075 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.

The EMBO journal ·Vol. 8 ·No. 10 ·1989-10-00 ·Pages 3113-9

Tyc K, Steitz JA

Abstract

Using anti-(U3)RNP autoantibodies, we have isolated and characterized two additional small nucleolar RNAs from HeLa cells, which are less abundant than U3 RNA. Both RNAs possess a trimethylguanosine cap as judged by precipitation with anti-TMG antibody, but are not precipitated by either anti-Sm or anti-La antibodies. In addition, both RNAs are not precipitable by anti-Th serum, which recognizes another nucleolar RNP autoantigen. Sequence analysis revealed that one of these RNAs, 136 nucleotides long, is the human U8 homolog; while the other, 105 nucleotides long, represents a novel species which we designate U13. Both RNAs share with U3 two conserved sequences (boxes C and D). The role of one or both of these boxes in binding the common 34 kd antigenic protein, otherwise known as fibrillarin, is discussed. Fractionation of HeLa cells revealed that U8 and U13, like U3, reside in the nucleolus. In glycerol gradients both RNAs cosediment with larger structures possibly representing ribosomal precursors. We propose that U3, U8 and U13 comprise a new subset of mammalian snRNPs whose roles in ribosome biogenesis are discussed.

MeSH Terms
Animals Base Sequence Cell Nucleolus/analysis Centrifugation, Density Gradient HeLa Cells Humans Mice Molecular Sequence Data Nucleic Acid Conformation Precipitin Tests Rats Ribonucleoproteins/analysis,classification,genetics Ribonucleoproteins, Small Nuclear Sequence Homology, Nucleic Acid
Chemicals
Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tyc K
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.
Steitz J A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-10-00
Pages
3113-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401391
Subset
IM
Grants
NIGMS NIH HHS · GM26154 · United States
External Links
PubMed source PMC Full Text View journal details
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