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PMID: 10811621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A bacterial sensor of plant cell contact controls the transcriptional induction of Ralstonia solanacearum pathogenicity genes.

The EMBO journal ·Vol. 19 ·No. 10 ·2000-05-15 ·Pages 2304-14

Aldon D, Brito B, Boucher C, Genin S

Abstract

The hrp genes of the plant pathogen Ralstonia solanacearum are key pathogenicity determinants; they encode a type III protein secretion machinery involved in the secretion of mediators of the bacterium-plant interaction. These hrp genes are under the genetic control of the hrpB regulatory gene, expression of which is induced when bacteria are co-cultivated with plant cell suspensions. In this study, we used hrp-gfp transcriptional fusions to demonstrate that the expression of the hrpB and type III secretion genes is specifically induced in response to the bacterium-plant cell contact. This contact-dependent induction of hrpB gene expression requires the outer membrane protein PrhA, but not a functional type III secretion apparatus. Genetic evidence indicates that PrhA constitutes the first example of a bacterial receptor for a non-diffusible signal present in the plant cell wall and which triggers the transcriptional activation of bacterial virulence genes.

MeSH Terms
Arabidopsis/genetics,microbiology Arabidopsis Proteins Bacterial Proteins/genetics Betaproteobacteria/pathogenicity,physiology DNA-Binding Proteins Gene Expression Regulation, Bacterial Homeodomain Proteins/genetics Plant Proteins/genetics Repressor Proteins/genetics Transcription Factors Transcription, Genetic
Chemicals
Arabidopsis Proteins Bacterial Proteins DNA-Binding Proteins Homeodomain Proteins PRHA protein, Arabidopsis Plant Proteins Repressor Proteins Transcription Factors hrpB protein, Ralstonia solanacearum
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aldon D
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326 Castanet-Tolosan Cedex, France.
Brito B
Boucher C
Genin S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.10.2304
Published
2000-05-15
Pages
2304-14
Language
English
Region
England
NLM ID
8208664
PMCID
PMC384368
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.10.2304 PMC Full Text View journal details
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