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PMID: 8026465 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Role for the outer membrane ferric siderophore receptor PupB in signal transduction across the bacterial cell envelope.

The EMBO journal ·Vol. 13 ·No. 12 ·1994-06-15 ·Pages 2805-13

Koster M, van Klompenburg W, Bitter W, Leong J, Weisbeek P

Abstract

The outer membrane protein PupB of Pseudomonas putida WCS358 facilitates transport of iron complexed to the siderophores pseudobactin BN8 and pseudobactin BN7 into the cell. Its synthesis is induced by the presence of these specific siderophores under iron limitation. The signal transduction pathway regulating siderophore-dependent expression of pupB was shown to consist of two regulatory proteins, PupI and PupR, and the PupB receptor itself. Mutational analysis of the regulatory genes suggested that PupI acts as a positive regulator of pupB transcription, whereas PupR modifies PupI activity dependent on the presence of pseudobactin BN8. PupI and PupR do not share homology with the classical bacterial two-component systems but display significant similarity to the FecI and FecR proteins of Escherichia coli involved in regulation of ferric dicitrate transport. The function of the PupB receptor in pupB regulation was studied by the use of chimeric receptor proteins composed of PupB and the ferric pseudobactin 358 receptor PupA. This experiment revealed that PupB is involved in the initiation of the signal transduction pathway, implying a so far unique role for an outer membrane protein in signal transduction.

Related Genes
pupB pupI pupR
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA-Binding Proteins/genetics Gene Expression Regulation, Bacterial Membrane Proteins/genetics Models, Genetic Molecular Sequence Data Pseudomonas putida/genetics Receptors, Cell Surface/genetics,metabolism Recombinant Fusion Proteins/metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction/genetics Transcription Factors/genetics
Chemicals
Bacterial Proteins DNA-Binding Proteins Membrane Proteins PupI protein, Pseudomonas putida PupR protein, Pseudomonas putida Receptors, Cell Surface Recombinant Fusion Proteins Transcription Factors pseudobactin receptor, Pseudomonas
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Koster M
University of Utrecht, Department of Molecular Cell Biology, The Netherlands.
van Klompenburg W
Bitter W
Leong J
Weisbeek P
References (39)
39 references, click to expand
  1. Microbial iron compounds.
    Annu Rev Biochem. 1981;50:715-31 PMID: 6455965
  2. Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp.
    J Bacteriol. 1986 Feb;165(2):585-94 PMID: 3003032
  3. Microbial envelope proteins related to iron.
    Annu Rev Microbiol. 1982;36:285-309 PMID: 6293371
  4. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  5. Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor.
    J Bacteriol. 1987 Jun;169(6):2624-30 PMID: 3294800
  6. Genetic organization and transcriptional analysis of a major gene cluster involved in siderophore biosynthesis in Pseudomonas putida WCS358.
    J Bacteriol. 1988 Apr;170(4):1812-9 PMID: 2450869
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  9. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  10. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression.
    J Bacteriol. 1988 May;170(5):2283-6 PMID: 2896188
  11. Genetics of the iron dicitrate transport system of Escherichia coli.
    J Bacteriol. 1988 Jun;170(6):2716-24 PMID: 2836368
  12. Molecular cloning, heterologous expression, and primary structure of the structural gene for the copper enzyme nitrous oxide reductase from denitrifying Pseudomonas stutzeri.
    J Bacteriol. 1988 Oct;170(10):4658-68 PMID: 3049543
  13. Cloning and characterization of a gene encoding an outer membrane protein required for siderophore-mediated uptake of Fe3+ in Pseudomonas putida WCS358.
    J Bacteriol. 1989 May;171(5):2819-26 PMID: 2540157
  14. Unorthodox expression of an enzyme: evidence for an untranslated region within carA from Pseudomonas aeruginosa.
    J Bacteriol. 1990 Feb;172(2):630-42 PMID: 2153657
  15. Prokaryotic signal transduction mediated by sensor and regulator protein pairs.
    Annu Rev Genet. 1989;23:311-36 PMID: 2694934
  16. Efficient DNA transformation of Bradyrhizobium japonicum by electroporation.
    Appl Environ Microbiol. 1990 Apr;56(4):833-6 PMID: 2187405
  17. A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria.
    Gene. 1990 Apr 30;89(1):37-46 PMID: 2115488
  18. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences.
    Nucleic Acids Res. 1990 Sep 11;18(17):5019-26 PMID: 2402433
  19. Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12.
    J Bacteriol. 1990 Dec;172(12):6749-58 PMID: 2254251
  20. Enterobactin-mediated iron transport in Pseudomonas aeruginosa.
    J Bacteriol. 1990 Dec;172(12):6991-6 PMID: 2174865
  21. TonB and the gram-negative dilemma.
    Mol Microbiol. 1990 Dec;4(12):2019-25 PMID: 2150975
  22. The ferric-pseudobactin receptor PupA of Pseudomonas putida WCS358: homology to TonB-dependent Escherichia coli receptors and specificity of the protein.
    Mol Microbiol. 1991 Mar;5(3):647-55 PMID: 1646376
  23. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule.
    J Mol Biol. 1992 May 20;225(2):487-94 PMID: 1593632
  24. Formation of a gated channel by a ligand-specific transport protein in the bacterial outer membrane.
    Science. 1992 Oct 16;258(5081):471-5 PMID: 1411544
  25. Identification and characterization of the exbB, exbD and tonB genes of Pseudomonas putida WCS358: their involvement in ferric-pseudobactin transport.
    Mol Microbiol. 1993 Jan;7(1):117-30 PMID: 8437515
  26. Signal transduction schemes of bacteria.
    Cell. 1993 Jun 4;73(5):857-71 PMID: 8098993
  27. Identification and characterization of the pupB gene encoding an inducible ferric-pseudobactin receptor of Pseudomonas putida WCS358.
    Mol Microbiol. 1993 May;8(3):591-601 PMID: 8392140
  28. Conversion of the FhuA transport protein into a diffusion channel through the outer membrane of Escherichia coli.
    EMBO J. 1993 Aug;12(8):3007-16 PMID: 7688295
  29. Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas aeruginosa: involvement of a two-component regulatory system.
    Mol Microbiol. 1993 Jun;8(6):1095-103 PMID: 8361354
  30. Identification and characterization of a siderophore regulatory gene (pfrA) of Pseudomonas putida WCS358: homology to the alginate regulatory gene algQ of Pseudomonas aeruginosa.
    Mol Microbiol. 1993 Oct;10(1):63-73 PMID: 7968519
  31. Two simple media for the demonstration of pyocyanin and fluorescin.
    J Lab Clin Med. 1954 Aug;44(2):301-7 PMID: 13184240
  32. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  33. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  34. A rapid alkaline extraction method for the isolation of plasmid DNA.
    Methods Enzymol. 1983;100:243-55 PMID: 6353143
  35. Exogenous induction of the iron dicitrate transport system of Escherichia coli K-12.
    J Bacteriol. 1984 Jul;159(1):271-7 PMID: 6376472
  36. Structure of pseudobactin 7SR1, a siderophore from a plant-deleterious Pseudomonas.
    Biochemistry. 1984 Jul 17;23(15):3534-40 PMID: 6466652
  37. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  38. Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358.
    J Bacteriol. 1985 Nov;164(2):563-70 PMID: 2997118
  39. Citrate-dependent iron transport system in Escherichia coli K-12.
    Eur J Biochem. 1981 Jul;117(2):431-7 PMID: 6268411
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-06-15
Pages
2805-13
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395160
Subset
IM
Databases
GENBANK
X77918
External Links
PubMed source PMC Full Text View journal details
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