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PMID: 2450869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic organization and transcriptional analysis of a major gene cluster involved in siderophore biosynthesis in Pseudomonas putida WCS358.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1812-9

Marugg JD, Nielander HB, Horrevoets AJ, van Megen I, van Genderen I, Weisbeek PJ

Abstract

In iron-limited environments, the plant-growth-stimulating Pseudomonas putida WCS358 produces a yellow-green fluorescent siderophore called pseudobactin 358. The transcriptional organization and the iron-regulated expression of a major gene cluster involved in the biosynthesis and transport of pseudobactin 358 were analyzed in detail. The cluster comprises a region with a minimum length of 33.5 kilobases and contains at least five transcriptional units, of which some are relatively large. The directions of transcription of four transcriptional units were determined by RNA-RNA hybridization and by analysis in Escherichia coli minicells. The latter also demonstrated that large polypeptides were encoded by these transcriptional units. The results allowed us to localize several promoter regions on the DNA. The iron-dependent expression of at least two genes within this cluster appears to be regulated at the transcriptional level.

MeSH Terms
DNA Restriction Enzymes DNA Transposable Elements Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Ionophores/genetics Mutation Nucleic Acid Hybridization Oligopeptides/biosynthesis,genetics Plasmids Promoter Regions, Genetic Protein Biosynthesis Pseudomonas/genetics RNA, Bacterial/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Ionophores Oligopeptides RNA, Bacterial pseudobactin DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marugg J D
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
Nielander H B
Horrevoets A J
van Megen I
van Genderen I
Weisbeek P J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1812-1819.1988
Published
1988-04-00
Pages
1812-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211035
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.4.1812-1819.1988 PMC Full Text View journal details
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