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PMID: 1082539 Published · ppublish English Journal Article

Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA.

Molecular & general genetics : MGG ·Vol. 143 ·No. 3 ·1976-02-02 ·Pages 279-90

von Gabain A, Hayward GS, Bujard H

Abstract

The DNA of bacteriophage T5+ (molecular weight 76 X 10(6) dalton) has been dissected by various specific endonucleases. The restriction enzymes HindIII and EcoRI produce 16 and 7 fragments respectively, whereas Sal and Sma produce 4 fragments each. Complete cleavage maps were established for the enzymes EcoRI, Sal and Sma and an almost complete map for HindIII. Furthermore the location and size of the deletions St 20, St 14, b3, St 0 and b1 were determined. The correlation of the genetic and functional map of the phage with the arrangement of fragments produced by the different enzymes has been established.

MeSH Terms
Binding Sites Chromosome Mapping Coliphages/chemistry,genetics DNA, Viral/analysis Electrophoresis, Agar Gel Endonucleases/metabolism Genotype Haemophilus influenzae/chemistry,genetics Mutation Nucleic Acid Denaturation Streptomyces/chemistry,genetics Time Factors
Chemicals
DNA, Viral Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
von Gabain A
Hayward G S
Bujard H
References (29)
29 references, click to expand
  1. Simplified quantitative electron microscopy of biopolymers.
    Biopolymers. 1970;9(3):373-9 PMID: 5417647
  2. Dual role of gene D5 in the development of bacteriophage T5.
    Nature. 1974 Feb 22;247(5442):554-6 PMID: 4594438
  3. Patterns of protein synthesis in T5-infected Escherichia coli.
    J Biol Chem. 1968 May 25;243(10):2550-9 PMID: 4870740
  4. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5.
    J Virol. 1971 May;7(5):612-8 PMID: 16789131
  5. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.
    J Biol Chem. 1964 Jan;239:222-32 PMID: 14114848
  6. Heteroduplex mapping of heat-resistant deletion mutants of bacteriophage t5.
    J Virol. 1975 May;15(5):1276-80 PMID: 16789160
  7. Structure and function of the genome of coliphage T5. 1. The physical structure of the chromosome of T5 + .
    Eur J Biochem. 1973 Mar 15;33(3):517-28 PMID: 4571500
  8. The calcium requirement of coliphage T5.
    J Immunol. 1949 Aug;62(4):505-16 PMID: 18133631
  9. Cleavage of T5 DNA by the Escherichia coli R-I restriction endonuclease.
    Virology. 1975 Mar;64(1):170-9 PMID: 1090074
  10. Analysis of the interruptions in bacteriophage T5 DNA.
    J Mol Biol. 1970 Feb 14;47(3):477-93 PMID: 5418167
  11. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.
    Nature. 1973 Jan 12;241(5385):120-3 PMID: 4572006
  12. Isolation of large molecular weight DNA from agarose gels for further digestion by restriction enzymes.
    FEBS Lett. 1975 Apr 15;53(1):84-6 PMID: 1140401
  13. Methionine and formylmethionine specific tRNAs coded by bacteriophage T5.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):558-62 PMID: 1091927
  14. Terminal repetition in the DNA of bacteriophage T5.
    J Mol Biol. 1972 Aug 21;69(2):187-200 PMID: 4560946
  15. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
    J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
  16. Regulation of expression of late genes of bacteriophage T5.
    J Virol. 1974 Jan;13(1):85-93 PMID: 4359432
  17. Structure and function of the genome of coliphage T5. 2. Regions of transcription of the chromosome.
    Eur J Biochem. 1973 Mar 15;33(3):529-34 PMID: 4693843
  18. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  19. The chromosome of bacteriophage T5. II. Arrangement of the single-stranded DNA fragments in the T5 + and T5st(O) chromosomes.
    J Mol Biol. 1972 Feb 14;63(3):397-407 PMID: 5014926
  20. Requirement of a phage-induced 5'-exonuclease for the expression of late genes of bacteriophage T5.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3502-5 PMID: 4357874
  21. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.
    J Mol Biol. 1972 Feb 14;63(3):383-95 PMID: 5014925
  22. Patterns of RNA synthesis in T5-infected cells. I. As studied by the technique of DNA-RNA hybridization-competition.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1249-56 PMID: 4916923
  23. Location of single-strand interruptions in the DNA of bacteriophage T5.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1167-74 PMID: 5256414
  24. The chromosome of bacteriophage T5. 3. Patterns of transcription from the single-stranded DNA fragments.
    J Mol Biol. 1973 Oct 25;80(2):345-59 PMID: 4763989
  25. A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.
    J Biol Chem. 1964 Jan;239:251-8 PMID: 14114851
  26. Properties of bacteriophage PM2: a lipid-containing bacterial virus.
    Virology. 1968 Apr;34(4):738-47 PMID: 5655723
  27. Electron microscopy of single-stranded DNA.
    J Mol Biol. 1970 Apr 14;49(1):125-37 PMID: 5450511
  28. Heat-stable mutants of T5 phage. I. The physical properties of the phage and their DNA molecules.
    Virology. 1968 Nov;36(3):356-76 PMID: 4881031
  29. Density mutants of phage T5.
    Virology. 1962 Dec;18:576-81 PMID: 13954145
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269404
Published
1976-02-02
Pages
279-90
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269404
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