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PMID: 1083417 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes.

The Journal of experimental medicine ·Vol. 143 ·No. 5 ·1976-05-01 ·Pages 1055-65

Vitetta ES, Forman J, Kettman JR

Abstract

Three populations of murine splenic B lymphocytes have been characterized previously (6, 7, 9) as those bearing only IgM, those bearing only IgD, and a population bearing both isotopes. These studies were designed to test the response of the IgM+ cells (IgM-only or IgM plus IgD) vs. the IgD-only cells to the B-cell mitogen, lipopolysaccharide. Results that after 1-4 days of culture, in the presence of mitogen, the IgM+ cells enlarge and elaborate an IgM polyclonal response. The IgD-only cells, in contrast, do not exhibit an IgM polyclonal response, but instead undergo blastogenesis and proliferation.

MeSH Terms
Animals Antibodies, Anti-Idiotypic Antibody Formation B-Lymphocytes/cytology,immunology Cell Separation Complement System Proteins/metabolism Immunoglobulin D/analysis Immunoglobulin M/analysis,biosynthesis Lipopolysaccharides Lymphocyte Activation Mice Polysaccharides, Bacterial Protein Biosynthesis Receptors, Antigen, B-Cell/analysis
Chemicals
Antibodies, Anti-Idiotypic Immunoglobulin D Immunoglobulin M Lipopolysaccharides Polysaccharides, Bacterial Receptors, Antigen, B-Cell Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vitetta E S
Forman J
Kettman J R
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22 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.5.1055
Published
1976-05-01
Pages
1055-65
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190195
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.5.1055 PMC Full Text View journal details
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