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PMID: 1084410 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro.

The Journal of experimental medicine ·Vol. 144 ·No. 1 ·1976-07-01 ·Pages 69-78

Scott DW

Abstract

A new method for the isolation of specific immunocompetent lymphocytes has been described in which lymphocyte populations are exposed to fluoresceinated antigens (FLAGs) in vivo or in vitro, and the FLAG-binding cells retained on antifluorescein affinity columns. Specific cells are then eluted with an unrelated FL-labeled protein and shown to be fully immunocompetent. This methodology has been applied successfully in diverse antigenic systems including polymerized flagellin and TNP-specific B cells and alloantigen-reactive cytotoxic T lymphocytes. The method is rapid, inexpensive (requiring only antifluorescein beads), and can be applied to any antigens (or antibodies) in which a fluorescein group can be introduced.

MeSH Terms
Animals Antigens B-Lymphocytes/analysis Cell Separation/methods Chromatography, Affinity Cytotoxicity Tests, Immunologic Fluoresceins Haptens Lymphocytes/analysis,immunology Male Mice Mice, Inbred Strains T-Lymphocytes/analysis
Chemicals
Antigens Fluoresceins Haptens
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Scott D W
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24 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.144.1.69
Published
1976-07-01
Pages
69-78
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190368
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.144.1.69 PMC Full Text View journal details
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