Hidden' IgG antiglobulins in normal human serum.

Clinical and experimental immunology ·Vol. 25 ·No. 2 ·1976-08-00 ·Pages 185-90

Hay FC, Nineham LJ, Torrigiani G, Roitt IM

Abstract

Hidden antiglobulins reacting with whole rabbit immunoglobulin were found by radioassay or indirect haemagglutination after gel filtration or ultracentrifugation of normal serum. Suprisingly, both the IgM and IgG antiglobulins were present in the same macroglobulin fractions. The IgG antiglobulins could be dissociated into 7S components by separating the serum under acid conditions. Antiglobulin activity was a function of the Fab region.

MeSH Terms

Antibodies, Anti-Idiotypic/analysis Antigen-Antibody Complex Binding Sites, Antibody Chromatography, Gel Hemagglutination Tests Humans Immunoglobulin Fab Fragments Immunoglobulin G/analysis Immunoglobulin M/analysis Molecular Weight

Chemicals

Antibodies, Anti-Idiotypic Antigen-Antibody Complex Immunoglobulin Fab Fragments Immunoglobulin G Immunoglobulin M

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hay F C

Nineham L J

Torrigiani G

Roitt I M

References (11)

11 references, click to expand

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Article Info

Journal

Clinical and experimental immunology

Abbr.

Clin Exp Immunol

ISSN

0009-9104

Published

1976-08-00

Pages

185-90

Language

English

Region

England

NLM ID

0057202

PMCID

PMC1541363

Subset

External Links

PubMed source PMC Full Text View journal details

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