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PMID: 10858232 Published · ppublish English Comparative Study Journal Article

Comparative genomics of Helicobacter pylori: analysis of the outer membrane protein families.

Infection and immunity ·Vol. 68 ·No. 7 ·2000-07-00 ·Pages 4155-68

Alm RA, Bina J, Andrews BM, Doig P, Hancock RE, Trust TJ

Abstract

The two complete genomic sequences of Helicobacter pylori J99 and 26695 were used to compare the paralogous families (related genes within one genome, likely to have related function) of genes predicted to encode outer membrane proteins which were present in each strain. We identified five paralogous gene families ranging in size from 3 to 33 members; two of these families contained members specific for either H. pylori J99 or H. pylori 26695. Most orthologous protein pairs (equivalent genes between two genomes, same function) shared considerable identity between the two strains. The unusual set of outer membrane proteins and the specialized outer membrane may be a reflection of the adaptation of H. pylori to the unique gastric environment where it is found. One subfamily of proteins, which contains both channel-forming and adhesin molecules, is extremely highly related at the sequence level and has likely arisen due to ancestral gene duplication. In addition, the largest paralogous family contained two essentially identical pairs of genes in both strains. The presence and genomic organization of these two pairs of duplicated genes were analyzed in a panel of independent H. pylori isolates. While one pair was present in every strain examined, one allele of the other pair appeared partially deleted in several isolates.

MeSH Terms
Alleles Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Base Sequence Binding Sites/genetics Conserved Sequence DNA Primers/genetics Evolution, Molecular Gene Duplication Genome, Bacterial Helicobacter pylori/classification,genetics,metabolism Humans Molecular Sequence Data Multigene Family Phylogeny Protein Sorting Signals/genetics Ribosomes/metabolism Sequence Homology, Amino Acid Species Specificity
Chemicals
Bacterial Outer Membrane Proteins DNA Primers Protein Sorting Signals
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Alm R A
Infection Discovery AstraZeneca R & D Boston, Waltham, Massachusetts 02451, USA. [email protected]
Bina J
Andrews B M
Doig P
Hancock R E
Trust T J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.7.4155-4168.2000
Email
[email protected]
Published
2000-07-00
Pages
4155-68
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC101716
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.7.4155-4168.2000 PMC Full Text View journal details
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