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PMID: 108668 Published · ppublish English Journal Article

Partial mapping of methylated sequences in Xenopus laevis ribosomal RNA by preparative hybridization to cloned fragments of ribosomal DNA.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 817-30

Maden BE, Reeder RH

Abstract

Xenopus laevis rRNA was hybridised to either of two cloned fragments of ribosomal DNA. One fragment, designated X1r11, contains a short region of the 18 S rRNA gene and most of the 28 S rRNA gene. The other fragment, X1r14, contains a short region of the 28 S gene and most of the 18 S gene. After hybridization the non-complementary rRNA was removed by digestion with T1 RNase and the hybridized RNA was then eluted and examined by fingerprinting analysis. The 3' terminal sequence and the dimethyl-A-containing sequence of 18 S rRNA both hybridized to X1r11 rDNA, in agreement with the known direction of transcription of rDNA. The distribution of other methylated oligonucleotides between the various fingerprints permitted assigment of nearly all of the methylated sequences in 18 S and 28 s rRNA to either the short 3' region or the long 5' region of the respective molecules.

MeSH Terms
Animals Base Sequence DNA, Recombinant Methylation Nucleic Acid Hybridization Oligoribonucleotides/analysis RNA, Ribosomal Ribonuclease T1 Ribonucleotides/analysis Ribosomes/analysis Xenopus
Chemicals
DNA, Recombinant Oligoribonucleotides RNA, Ribosomal Ribonucleotides Ribonuclease T1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maden B E
Reeder R H
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.3.817
Published
1979-03-00
Pages
817-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327736
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.3.817 PMC Full Text View journal details
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