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PMID: 10872324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and characterization of Xenopus laevis topoisomerase II.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1303-8

Benedetti P, Baldi MI, Mattoccia E, Tocchini-Valentini GP

Abstract

We have purified to apparent homogeneity a type II DNA topoisomerase from Xenopus laevis oocyte nuclei (germinal vesicles, or GV). The most pure preparations contain a single polypeptide of 175,000 daltons as determined by SDS-gel electrophoresis. The enzyme changes the linking number of DNA circles in steps of two and reversibly knots or catenates DNA rings. No gyrase activity is detectable and ATP is required.

MeSH Terms
Animals Catalysis Cellulose/analogs & derivatives Chromatography/methods Chromatography, Affinity DNA Topoisomerases, Type II/isolation & purification,metabolism Dextrans Enzyme Stability Peptides Topoisomerase II Inhibitors Xenopus laevis
Chemicals
Dextrans Peptides Topoisomerase II Inhibitors Cellulose sephadex phosphocellulose DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Benedetti P
Istituto di Biologica Cellulare, CNR, Rome, Italy.
Baldi M I
Mattoccia E
Tocchini-Valentini G P
References (25)
25 references, click to expand
  1. DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4551-4 PMID: 188045
  2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  3. Proteins that affect DNA conformation.
    Annu Rev Biochem. 1978;47:449-79 PMID: 209728
  4. Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4838-42 PMID: 368801
  5. A sign inversion mechanism for enzymatic supercoiling of DNA.
    Science. 1979 Nov 30;206(4422):1081-3 PMID: 227059
  6. Separation of RNA transcription and processing activities from X. laevis germinal vesicles.
    Cell. 1979 Nov;18(3):643-8 PMID: 519751
  7. DNA gyrase and the supercoiling of DNA.
    Science. 1980 Feb 29;207(4434):953-60 PMID: 6243420
  8. Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break.
    Cell. 1980 Mar;19(3):697-707 PMID: 6244895
  9. DNA gyrase action involves the introduction of transient double-strand breaks into DNA.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1847-51 PMID: 6246508
  10. Formation and resolution of DNA catenanes by DNA gyrase.
    Cell. 1980 May;20(1):245-54 PMID: 6248235
  11. In vitro catenation and decatenation of DNA and a novel eucaryotic ATP-dependent topoisomerase.
    Cell. 1980 Jun;20(2):461-7 PMID: 6248247
  12. ATP-dependent DNA topoisonmerase from D. melanogaster reversibly catenates duplex DNA rings.
    Cell. 1980 Aug;21(1):115-25 PMID: 6250707
  13. DNA topoisomerases.
    Cell. 1980 Nov;22(2 Pt 2):327-8 PMID: 6256077
  14. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  15. Purification and characterization of Xenopus laevis type I topoisomerase.
    J Biol Chem. 1981 Apr 25;256(8):3654-61 PMID: 6260773
  16. Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme).
    J Biol Chem. 1981 Jun 10;256(11):5860-5 PMID: 6263892
  17. A homogeneous type II DNA topoisomerase from HeLa cell nuclei.
    J Biol Chem. 1981 Sep 10;256(17):9334-9 PMID: 6267071
  18. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  19. DNA gyrase: affinity chromatography on novobiocin-Sepharose and catalytic properties.
    Nucleic Acids Res. 1981 Aug 11;9(15):3589-603 PMID: 6269086
  20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  21. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6 PMID: 4333036
  22. An omega protein from Drosophila melanogaster.
    Biochemistry. 1974 Oct 8;13(21):4299-303 PMID: 4212937
  23. Characterization of purified DNA-relaxing enzyme from human tissue culture cells.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2550-4 PMID: 170605
  24. Purification and characterization of the DNA untwisting enzyme from rat liver.
    Biochemistry. 1976 Oct 19;15(21):4638-42 PMID: 184821
  25. Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.
    Chromosoma. 1978 May 16;66(4):299-308 PMID: 657930
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1303-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555276
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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