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PMID: 10884414 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two mechanisms for termination of individual Ca2+ sparks in skeletal muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 14 ·2000-07-05 ·Pages 7823-8

Lacampagne A, Klein MG, Ward CW, Schneider MF

Abstract

Ca(2+) sparks are brief, localized elevations of myoplasmic [Ca(2+)] caused by release of increments of Ca(2+) via sarcoplasmic reticulum Ca(2+) release channels in muscle. The properties of individual sparks provide information regarding the opening of sarcoplasmic reticulum Ca(2+) channels within functioning cells. Here we use high-speed confocal microscopy to show that individual Ca(2+) sparks activated by membrane depolarization in single frog skeletal muscle fibers can be terminated prematurely by repolarization. Thus, either voltage sensor deactivation on repolarization or release channel inactivation during continued depolarization can terminate the Ca(2+) release channel activity underlying voltage-activated Ca(2+) sparks in skeletal muscle.

MeSH Terms
Aniline Compounds Animals Anura Calcium/metabolism Calcium Channels/metabolism Calcium Channels, L-Type/metabolism Membrane Potentials Microscopy, Confocal Microscopy, Video Models, Biological Models, Molecular Muscle Fibers, Skeletal/metabolism Muscle, Skeletal/metabolism Patch-Clamp Techniques Ryanodine Receptor Calcium Release Channel/metabolism Sarcoplasmic Reticulum/metabolism Xanthenes
Chemicals
Aniline Compounds Calcium Channels Calcium Channels, L-Type Ryanodine Receptor Calcium Release Channel Xanthenes Fluo-3 Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lacampagne A
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA.
Klein M G
Ward C W
Schneider M F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.97.14.7823
Published
2000-07-05
Pages
7823-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16629
Subset
IM
Grants
NIAMS NIH HHS · R01 AR044197 · United States
NINDS NIH HHS · R01 NS023346 · United States
NIAMS NIH HHS · AR44197 · United States
NINDS NIH HHS · NS23346 · United States
External Links
PubMed source DOI: 10.1073/pnas.97.14.7823 PMC Full Text View journal details
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