Construction and use of derivatives of transposon Tn4001 that function in Mycoplasma pulmonis and Mycoplasma arthritidis.
Previous attempts to introduce transposon Tn4001 into Mycoplasma pulmonis and Mycoplasma arthritidis have not been successful, possibly due to functional failure of the transposon's gentamicin resistance determinant. Tn4001C and Tn4001T were constructed, respectively, by insertion of a chloramphenicol acetyltransferase gene and the tetM tetracycline resistance determinant into Tn4001. Both Tn4001C and Tn4001T transposed in M. pulmonis, and Tn4001T transposed in M. arthritidis. The incorporation of a Tn4001T derivative that contained lacZ into either Mycoplasma species resulted in transformants with readily detectable LacZ activity. Tn4001T may be of general utility for use as a mycoplasma cloning vehicle because tetM functions in all species of Mycoplasma examined thus far.
21 references, click to expand
-
Gene transfer in Mycoplasma arthritidis: transformation, conjugal transfer of Tn916, and evidence for a restriction system recognizing AGCT.J Bacteriol. 1996 Oct;178(20):6078-81 PMID: 8830712
-
Mechanism of antigenic variation in Mycoplasma pulmonis: interwoven, site-specific DNA inversions.Mol Microbiol. 1995 Nov;18(4):703-14 PMID: 8817492
-
A family of phase-variable restriction enzymes with differing specificities generated by high-frequency gene rearrangements.Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13923-8 PMID: 9811902
-
Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development.J Bacteriol. 1999 Feb;181(4):1079-87 PMID: 9973332
-
Construction and analysis of a modified Tn4001 conferring chloramphenicol resistance in Mycoplasma pneumoniae.Plasmid. 1999 Mar;41(2):120-4 PMID: 10087215
-
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
-
Isolation of Mycoplasma pulmonis membranes and identification of surface antigens.Infect Immun. 1987 May;55(5):1314-20 PMID: 3570466
-
Transformation of Mycoplasma pulmonis and Mycoplasma hyorhinis: transposition of Tn916 and formation of cointegrate structures.Plasmid. 1988 Jul;20(1):33-41 PMID: 2853878
-
Random insertion of the gentamicin resistance transposon Tn4001 in Mycoplasma pulmonis.Plasmid. 1989 Jan;21(1):43-7 PMID: 2543007
-
Cloning and DNA sequence of a mycoplasmal recA gene.J Bacteriol. 1992 Feb;174(3):778-84 PMID: 1732213
-
High-frequency rearrangements in the chromosome of Mycoplasma pulmonis correlate with phenotypic switching.Mol Microbiol. 1992 May;6(9):1149-54 PMID: 1350316
-
Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis.Microbiol Immunol. 1992;36(6):661-5 PMID: 1522817
-
Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction.J Clin Microbiol. 1993 May;31(5):1358-61 PMID: 7684753
-
The genetics and basic biology of Mycoplasma pulmonis: how much is actually Acholeplasma?Plasmid. 1993 Sep;30(2):176-8 PMID: 8234493
-
Construction of Tn4001lac derivatives to be used as promoter probe vectors in mycoplasmas.Gene. 1993 Dec 31;137(2):217-22 PMID: 8299950
-
Mycoplasmal cloning vectors derived from plasmid pKMK1.Plasmid. 1994 Jan;31(1):49-59 PMID: 8171125
-
Transformation of Mycoplasma capricolum and examination of DNA restriction modification in M. capricolum and Mycoplasma mycoides subsp. mycoides.Plasmid. 1994 May;31(3):308-11 PMID: 8058824
-
High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme.J Biotechnol. 1994 Aug 15;36(2):145-55 PMID: 7765234
-
Association of lysogenic bacteriophage MAV1 with virulence of Mycoplasma arthritidis.Infect Immun. 1995 Oct;63(10):4016-23 PMID: 7558313
-
Sequence analysis of the chromosomal region around and within the V-1-encoding gene of Mycoplasma pulmonis: evidence for DNA inversion as a mechanism for V-1 variation.Infect Immun. 1996 Feb;64(2):472-9 PMID: 8550194
-
Molecular biology of mycoplasmas.Annu Rev Microbiol. 1996;50:25-57 PMID: 8905075
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