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PMID: 1089636 Published · ppublish English Journal Article

Positive selection of general amino acid permease mutants in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 121 ·No. 2 ·1975-02-00 ·Pages 562-70

Rytka J

Abstract

It was found that D-stereoisomers of natural amino acids inhibit the growth of Saccharomyces cerevisiae cells. Kinetic and genetic evidence showed that d-amino acids enter the cell by the general amino acid permease. Two classes of S. cerevisiae mutants resistant to d-amino acids were isolated. One class of mutants appeared to be defective in the general amino acid permease specified by the gene gap. In the second class, the activity of general amino acid permease was affected by ammonium ions. Mutants of the second class were isolated in a yeast strain with the general amino acid permease insensitive to the presence of ammonium ions in culture media. The mutation affecting the permease, amc, occurred in a locus unlinked to gap.

MeSH Terms
Amino Acids/metabolism Ammonium Sulfate/metabolism Biological Transport, Active Carbon Radioisotopes Diploidy Genetic Complementation Test Histidine/metabolism Membrane Transport Proteins/biosynthesis Mutation Proline/metabolism Saccharomyces cerevisiae/enzymology,growth & development,metabolism Serine/metabolism Stereoisomerism Tyrosine/metabolism
Chemicals
Amino Acids Carbon Radioisotopes Membrane Transport Proteins Tyrosine Serine Histidine Proline Ammonium Sulfate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rytka J
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.121.2.562-570.1975
Published
1975-02-00
Pages
562-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245967
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.121.2.562-570.1975 PMC Full Text View journal details
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