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PMID: 1089637 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Urea transport in Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 121 ·No. 2 ·1975-02-00 ·Pages 571-6

Cooper TG, Sumrada R

Abstract

Urea transport in Saccharomyces cerevisiae occurs by two pathways. The first mode of uptake is via an active transport system which: (i) has an apparent Km value of 14 muM, (ii) is absolutely dependent upon energy metabolism, (iii) requires pre-growth of the cultures in the presence of oxaluric acid, gratuitous inducer of the allantoin degradative enzymes, and (iv) is sensitive to nitrogen repression. The second mode of uptake which occurs at external urea concentrations in excess of 0.5 mM is via either passive or facilitated diffusion.

MeSH Terms

Ammonia/metabolism Arginine/metabolism Arsenates/pharmacology Aspartic Acid/metabolism Biological Transport, Active Cyanides/pharmacology Depression, Chemical Dinitrophenols/pharmacology Energy Metabolism Fluorides/pharmacology Hydrolases/biosynthesis Ligases/biosynthesis Mutation Proline/metabolism Saccharomyces cerevisiae/enzymology,metabolism Urea/metabolism Uric Acid/metabolism

Chemicals

Arsenates Cyanides Dinitrophenols Uric Acid Aspartic Acid Ammonia Urea Arginine Proline Hydrolases Ligases Fluorides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cooper T G

Sumrada R

References (12)

12 references, click to expand

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Lomofungin, an inhibitor of ribonucleic acid synthesis in yeast protoplasts: its effect on enzyme formation.
Antimicrob Agents Chemother. 1973 Jun;3(6):716-22 PMID: 4790623
Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae.
J Biol Chem. 1972 Mar 10;247(5):1349-53 PMID: 4551940
Mechanism of hydrolysis of O-nitrophenyl-beta-galactoside in Staphylococcus aureus and its significance for theories of sugar transport.
Proc Natl Acad Sci U S A. 1967 Jul;58(1):225-8 PMID: 5341056
Effects of colicins E1 and K on transport systems.
J Bacteriol. 1969 Jan;97(1):57-63 PMID: 4884823
Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae.
J Bacteriol. 1974 Mar;117(3):1240-7 PMID: 4591950
Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340-4 PMID: 4599622
Nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae.
J Bacteriol. 1974 Jun;118(3):821-9 PMID: 4598006
Urea carboxylase and allophanate hydrolase: two components of a multienzyme complex in Saccharomyces cerevisiae.
Biochem Biophys Res Commun. 1972 Oct 6;49(1):45-51 PMID: 4562164
The induction of urea carboxylase and allophanate hydrolase in Saccharomyces cerevisiae.
J Biol Chem. 1973 Sep 10;248(17):6203-9 PMID: 4580053
Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae.
Biochem Biophys Res Commun. 1973 Dec 19;55(4):1100-4 PMID: 4589302

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.121.2.571-576.1975

Published

1975-02-00

Pages

571-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245968

Subset

External Links

PubMed source DOI: 10.1128/jb.121.2.571-576.1975 PMC Full Text View journal details

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