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PMID: 1091618 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli.

Journal of bacteriology ·Vol. 122 ·No. 1 ·1975-04-00 ·Pages 120-8

Steege DA, Low B

Abstract

Specialized lambda transducing phages for the sul+ (supD-) amber suppressor in Escherichia coli K-12 have been isolated, using a secondary site lambda-cI857 lysogen in which we have shown the prophage to be closely linked to sul+.sul+ transducing particles were detected frequently, at 10-5 per plaque-forming unit, in lysates prepared from the secondary-site lysogens. High-frequency transducing lysates were obtained from several independently isolated sul+ transductants and were analyzed by CsCl equilibrium density gradient centrifugation. The transducing phages are defective; marker rescue analysis indicates that the lambda-N gene is not present. In lambda-cI857DELTANdSul+, a bio-type transducing phage, the genes specifying recombination and excision functions have been replaced by bacterial deoxyribonucleic acid.

MeSH Terms
Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Chromosome Mapping Coliphages/growth & development,isolation & purification DNA Viruses Defective Viruses/growth & development Escherichia coli/metabolism Genetic Linkage Mutagens Mutation Nitrosoguanidines RNA Viruses RNA, Bacterial RNA, Transfer Recombination, Genetic Suppression, Genetic Thymine Transduction, Genetic
Chemicals
Mutagens Nitrosoguanidines RNA, Bacterial RNA, Transfer Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Steege D A
Low B
References (42)
42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.122.1.120-128.1975
Published
1975-04-00
Pages
120-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235648
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.122.1.120-128.1975 PMC Full Text View journal details
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