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PMID: 1091630 Published · ppublish English Journal Article

Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor.

Journal of bacteriology ·Vol. 122 ·No. 1 ·1975-04-00 ·Pages 59-65

Beard JP, Connolly JC

Abstract

The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the pool is about one-half of the amount of protein incorporated into mature sex pili at the peak of production and is independent of the phase of growth of the culture. The molecular weight of the protein in the pool and of the subunit of the sex pili specified by the cells is 12,500 plus or minus 600.

MeSH Terms
Bacterial Proteins/analysis Cell Fractionation Cell Membrane/analysis Cell Wall/analysis Coliphages Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Escherichia coli/analysis,growth & development,ultrastructure Extrachromosomal Inheritance Genetic Variation Glycerol Molecular Weight RNA Viruses Sex Spheroplasts/analysis Tritium
Chemicals
Bacterial Proteins Tritium Glycerol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beard J P
Connolly J C
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.122.1.59-65.1975
Published
1975-04-00
Pages
59-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235639
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.122.1.59-65.1975 PMC Full Text View journal details
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