Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.

Journal of bacteriology ·Vol. 117 ·No. 2 ·1974-02-00 ·Pages 406-16

Ames GF, Spudich EN, Nikaido H

Abstract

The protein composition of the outer membrane of Salmonella typhimurium has been analyzed by electrophoresis on slabs of sodium dodecyl sulfate-acrylamide gel. This powerful technique allows very high resolution of protein mixtures and has permitted the identification of multiple major protein components of the outer membrane; no evidence for a single major component of molecular weight 44,000 was obtained. These proteins were shown to be decreased in amount in mutants which have defective lipopolysaccharides. Mutants of an apparently new type were also found which contain decreased amounts of the proteins and the parent-like lipopolysaccharide, yet are resistant to a lipopolysaccharide-specific phage, C21. Several outer membrane proteins are insoluble in sodium dodecyl sulfate unless heated at high temperature (above 70 C). A purification procedure based on this property is tentatively suggested.

MeSH Terms

Bacterial Proteins/analysis,isolation & purification Cell Wall/analysis Chromatography, Gas Chromatography, Gel Electrophoresis, Polyacrylamide Gel Glucosamine/analysis Heptoses/analysis Hot Temperature Keto Acids/analysis Lipopolysaccharides/analysis Molecular Weight Mutation Polysaccharides, Bacterial/analysis Salmonella typhimurium/analysis,cytology Solubility

Chemicals

Bacterial Proteins Heptoses Keto Acids Lipopolysaccharides Polysaccharides, Bacterial Glucosamine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ames G F

Spudich E N

Nikaido H

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25 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.117.2.406-416.1974

Published

1974-02-00

Pages

406-16

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285527

Subset

External Links

PubMed source DOI: 10.1128/jb.117.2.406-416.1974 PMC Full Text View journal details

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Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.

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