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PMID: 10921875 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vaccinia virus infection disrupts microtubule organization and centrosome function.

The EMBO journal ·Vol. 19 ·No. 15 ·2000-08-01 ·Pages 3932-44

Ploubidou A, Moreau V, Ashman K, Reckmann I, González C, Way M

Abstract

We examined the role of the microtubule cytoskeleton during vaccinia virus infection. We found that newly assembled virus particles accumulate in the vicinity of the microtubule-organizing centre in a microtubule- and dynein-dynactin complex-dependent fashion. Microtubules are required for efficient intracellular mature virus (IMV) formation and are essential for intracellular enveloped virus (IEV) assembly. As infection proceeds, the microtubule cytoskeleton becomes dramatically reorganized in a fashion reminiscent of overexpression of microtubule-associated proteins (MAPs). Consistent with this, we report that the vaccinia proteins A10L and L4R have MAP-like properties and mediate direct binding of viral cores to microtubules in vitro. In addition, vaccinia infection also results in severe reduction of proteins at the centrosome and loss of centrosomal microtubule nucleation efficiency. This represents the first example of viral-induced disruption of centrosome function. Further studies with vaccinia will provide insights into the role of microtubules during viral pathogenesis and regulation of centrosome function.

MeSH Terms
Centrosome/physiology Cytoskeleton/drug effects Dynactin Complex Dyneins/metabolism Fluorescent Antibody Technique Genome, Viral Microtubule-Associated Proteins/metabolism Microtubules/physiology Nocodazole/pharmacology Vaccinia virus/physiology Viral Proteins/isolation & purification,metabolism Virus Assembly
Chemicals
Dynactin Complex Microtubule-Associated Proteins Viral Proteins Dyneins Nocodazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ploubidou A
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Moreau V
Ashman K
Reckmann I
González C
Way M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.15.3932
Published
2000-08-01
Pages
3932-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC306617
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.15.3932 PMC Full Text View journal details
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