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PMID: 1093165 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromatin directed transcription of 5S and tRNA genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 1082-6

Marzluff WF, Huang RC

Abstract

Chromatin prepared by gentle methods from mouse myeloma cells retained its ability to synthesize RNA using bound endogenous RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). The transcription resembles that observed in vivo in several respects. The low-molecular-weight RNA species 5S RNA and the 4.5S precursor to 4S RNA, are transcribed accurately and transcription is reinitiated continually in vitro. Their synthesis was not inhibited by alpha-amanitin (1 mug/ml) as was found previously for these species in isolated nuclei.

MeSH Terms
Animals Cell Line Chromatin/metabolism DNA, Neoplasm/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Molecular Weight Myeloma Proteins Nucleic Acid Hybridization Nucleic Acid Precursors/biosynthesis RNA, Neoplasm/biosynthesis RNA, Ribosomal/biosynthesis RNA, Transfer/biosynthesis Transcription, Genetic Xenopus
Chemicals
Chromatin DNA, Neoplasm Myeloma Proteins Nucleic Acid Precursors RNA, Neoplasm RNA, Ribosomal RNA, Transfer DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Marzluff W F
Huang R C
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.1082
Published
1975-03-00
Pages
1082-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432470
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.1082 PMC Full Text View journal details
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