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PMID: 10931861 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphorylation of the vesicle-tethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments.

The Journal of cell biology ·Vol. 150 ·No. 3 ·2000-08-07 ·Pages 475-88

Dirac-Svejstrup AB, Shorter J, Waters MG, Warren G

Abstract

Coat protein I (COPI) transport vesicles can be tethered to Golgi membranes by a complex of fibrous, coiled-coil proteins comprising p115, Giantin and GM130. p115 has been postulated to act as a bridge, linking Giantin on the vesicle to GM130 on the Golgi membrane. Here we show that the acidic COOH terminus of p115 mediates binding to both GM130 and Giantin as well as linking the two together. Phosphorylation of serine 941 within this acidic domain enhances the binding as well as the link between them. Phosphorylation is mediated by casein kinase II (CKII) or a CKII-like kinase. Surprisingly, the highly conserved NH(2)-terminal head domain of p115 is not required for the NSF (N-ethylmaleimide-sensitive fusion protein)-catalyzed reassembly of cisternae from mitotic Golgi fragments in a cell-free system. However, the ability of p115 to link GM130 to Giantin and the phosphorylation of p115 at serine 941 are required for NSF-catalyzed cisternal regrowth. p115 phosphorylation may be required for the transition from COPI vesicle tethering to COPI vesicle docking, an event that involves the formation of trans-SNARE [corrected] (trans-soluble NSF attachment protein [SNAP] receptor) complexes.

MeSH Terms
Amino Acid Sequence Autoantigens Binding Sites Carrier Proteins/metabolism Casein Kinase II Golgi Apparatus/physiology Golgi Matrix Proteins Membrane Fusion Membrane Proteins/metabolism Mitosis/physiology Molecular Sequence Data N-Ethylmaleimide-Sensitive Proteins Phosphorylation Protein Binding Protein Serine-Threonine Kinases/antagonists & inhibitors,metabolism Serine/metabolism Vesicular Transport Proteins
Chemicals
Autoantigens Carrier Proteins Golgi Matrix Proteins Golgin subfamily A member 2 Membrane Proteins Vesicular Transport Proteins macrogolgin vesicular transport factor p115 Serine Casein Kinase II Protein Serine-Threonine Kinases N-Ethylmaleimide-Sensitive Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dirac-Svejstrup A B
Cell Biology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom.
Shorter J
Waters M G
Warren G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.150.3.475
Published
2000-08-07
Pages
475-88
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175190
Subset
IM
Grants
NIGMS NIH HHS · GM60478-01 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.150.3.475 PMC Full Text View journal details
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