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PMID: 8557739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a cis-Golgi matrix protein, GM130.

The Journal of cell biology ·Vol. 131 ·No. 6 Pt 2 ·1995-12-00 ·Pages 1715-26

Nakamura N, Rabouille C, Watson R, Nilsson T, Hui N, Slusarewicz P, Kreis TE, Warren G

Abstract

Antisera raised to a detergent- and salt-resistant matrix fraction from rat liver Golgi stacks were used to screen an expression library from rat liver cDNA. A full-length clone was obtained encoding a protein of 130 kD (termed GM130), the COOH-terminal domain of which was highly homologous to a Golgi human auto-antigen, golgin-95 (Fritzler et al., 1993). Biochemical data showed that GM130 is a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and part of a larger oligomeric complex. Predictions from the protein sequence suggest that GM130 is an extended rod-like protein with coiled-coil domains. Immunofluorescence microscopy showed partial overlap with medial- and trans-Golgi markers but almost complete overlap with the cis-Golgi network (CGN) marker, syntaxin5. Immunoelectron microscopy confirmed this location showing that most of the GM130 was located in the CGN and in one or two cisternae on the cis-side of the Golgi stack. GM130 was not re-distributed to the ER in the presence of brefeldin A but maintained its overlap with syntaxin5 and a partial overlap with the ER-Golgi intermediate compartment marker, p53. Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure.

MeSH Terms
Amino Acid Sequence Animals Autoantigens Base Sequence Cell Fractionation Cloning, Molecular Fluorescent Antibody Technique Gene Library Genetic Testing Golgi Apparatus/chemistry,genetics,ultrastructure Liver/cytology Membrane Proteins/analysis,chemistry,genetics,ultrastructure Molecular Sequence Data Rabbits Rats Sequence Analysis, DNA
Chemicals
Autoantigens Golgin subfamily A member 2 Membrane Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Nakamura N
Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK.
Rabouille C
Watson R
Nilsson T
Hui N
Slusarewicz P
Kreis T E
Warren G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.6.1715
Published
1995-12-00
Pages
1715-26
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120691
Subset
IM
Databases
GENBANK
U35022
External Links
PubMed source DOI: 10.1083/jcb.131.6.1715 PMC Full Text View journal details
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