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PMID: 10931870 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in synaptojanin disrupt synaptic vesicle recycling.

The Journal of cell biology ·Vol. 150 ·No. 3 ·2000-08-07 ·Pages 589-600

Harris TW, Hartwieg E, Horvitz HR, Jorgensen EM

Abstract

Synaptojanin is a polyphosphoinositide phosphatase that is found at synapses and binds to proteins implicated in endocytosis. For these reasons, it has been proposed that synaptojanin is involved in the recycling of synaptic vesicles. Here, we demonstrate that the unc-26 gene encodes the Caenorhabditis elegans ortholog of synaptojanin. unc-26 mutants exhibit defects in vesicle trafficking in several tissues, but most defects are found at synaptic termini. Specifically, we observed defects in the budding of synaptic vesicles from the plasma membrane, in the uncoating of vesicles after fission, in the recovery of vesicles from endosomes, and in the tethering of vesicles to the cytoskeleton. Thus, these results confirm studies of the mouse synaptojanin 1 mutants, which exhibit defects in the uncoating of synaptic vesicles (Cremona, O., G. Di Paolo, M.R. Wenk, A. Luthi, W.T. Kim, K. Takei, L. Daniell, Y. Nemoto, S.B. Shears, R.A. Flavell, D.A. McCormick, and P. De Camilli. 1999. Cell. 99:179-188), and further demonstrate that synaptojanin facilitates multiple steps of synaptic vesicle recycling.

MeSH Terms
Alleles Amino Acid Sequence Animals Biological Transport Caenorhabditis elegans/genetics Cloning, Molecular Cytoskeleton/ultrastructure Endocytosis Gene Dosage Genes, Helminth Molecular Sequence Data Mutation Nerve Tissue Proteins/genetics Phosphoric Monoester Hydrolases/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Synaptic Transmission/genetics Synaptic Vesicles/physiology,ultrastructure
Chemicals
Nerve Tissue Proteins synaptojanin Phosphoric Monoester Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Harris T W
Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840, USA.
Hartwieg E
Horvitz H R
Jorgensen E M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.150.3.589
Published
2000-08-07
Pages
589-600
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175188
Subset
IM
Grants
NIGMS NIH HHS · GM29663 · United States
NINDS NIH HHS · R01 NS 34307 · United States
Databases
GENBANK
AF283322, AF283323
External Links
PubMed source DOI: 10.1083/jcb.150.3.589 PMC Full Text View journal details
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