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PMID: 1094462 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ribosomal protein S1 and polypeptide chain initiation in bacteria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2325-9

Szer W, Hermoso JM, Leffler S

Abstract

Among several subspecies of 30S subunits of Escherichia coli observed by polyacrylamide-agarose gel electrophoresis, only the slow-moving, protein S1-containing subspecies participates in the formation of the 30S initiation complex with coliphage MS2 RNA as mRNA; the other subspecies retain activity with AUG as mRNA; they are also active in the poly(U)-directed binding of Phe-tRNA. Protein S1 from Caulobacter crescentus substitutes for E. coli S1 despite the fact that C. crescentus ribosomes do not bind MS2 RNA. Under appropriate conditions, the entire population of E. coli 30S subunits can be isolated as the S1-containing subspecies. Protein S1 is lost by salt treatment of ribosomes.

MeSH Terms
Bacteria Bacterial Proteins/metabolism Centrifugation, Density Gradient Electrophoresis Escherichia coli/drug effects,metabolism,ultrastructure Osmolar Concentration Peptide Chain Initiation, Translational Poly U/metabolism,pharmacology RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomal Proteins/metabolism Ribosomes/drug effects,metabolism
Chemicals
Bacterial Proteins RNA, Messenger Ribosomal Proteins Poly U RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Szer W
Hermoso J M
Leffler S
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2325
Published
1975-06-00
Pages
2325-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432750
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2325 PMC Full Text View journal details
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