Home LiteratureArticle Details
PMID: 1097214 Published · ppublish English Journal Article

In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts.

Chromosoma ·Vol. 50 ·No. 4 ·1975-00-00 ·Pages 349-69

Hennen S, Mizuno S, Macgregor HC

Abstract

Methods are described for in situ hybridization of ribosomal DNA from Xenopus laevis, labelled in vitro with 125iodine, to mitotic and lampbrush chromosomes from Triturus cristatus carnifex. The hybridization reaction was carried out in a mixture containing 50% formamide, 4 X SSC, 0.1 M KI, at 37 degrees C, or in 2 X SSC, 0.1 M KI at 65 degrees C. Autoradiographs of mitotic metaphases from 2 males showed labelling over the middle of the short arm of one chromosome IX in each metaphase. In some cases, a region near the end of a longer chromosome was also labelled. In a lampbrush preparations, labelling was confined to a region identified as about 53 units, near the middle of the short arm of both halves of bivalent IX. The usefulness of the technique and the significance of the labelling of only 1 of the 2 chromosomes IX in mitotic preparations are discussed.

MeSH Terms
Animals Autoradiography Centrifugation, Density Gradient Chromosomes DNA Female Iodine Radioisotopes Karyotyping Male Methods Microscopy, Phase-Contrast Mitosis Nucleic Acid Hybridization Ovary Ovum/cytology Ribosomes Triturus Xenopus
Chemicals
Iodine Radioisotopes DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hennen S
Mizuno S
Macgregor H C
References (37)
37 references, click to expand
  1. The nucleolus and its genes in amphibian oogenesis.
    Biol Rev Camb Philos Soc. 1972 May;47(2):177-210 PMID: 4587818
  2. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  3. Formation and detection of RNA-DNA hybrid molecules in cytological preparations.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):378-83 PMID: 4895535
  4. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER.
    Proc Natl Acad Sci U S A. 1965 Apr;53:737-45 PMID: 14324529
  5. Reptitive DNA sequences in drosophila.
    Chromosoma. 1971;33(3):319-44 PMID: 5088497
  6. Chromosome location of the genes for 28S, 18S and 5S ribosomal RNA in Triturus marmoratus (Amphibia Urodela).
    Chromosoma. 1974;49(2):135-53 PMID: 4614960
  7. Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes.
    Chromosoma. 1970 Dec 2;32(1):31-63 PMID: 5511945
  8. Satellite DNA associated with heterochromatin in Rhynchosciara.
    Chromosoma. 1971 Mar 16;32(4):407-27 PMID: 4102329
  9. Mutations affecting the size of the nucleolus in Xenopus laevis.
    Nature. 1970 Sep 12;227(5263):1108-10 PMID: 5451103
  10. The localization of transfer RNA5Lys genes in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3527-31 PMID: 4215081
  11. Clustering of transfer RNA genes of Xenopus laevis.
    J Mol Biol. 1973 Sep 15;79(2):411-29 PMID: 4760136
  12. A quick-freeze method for making smear slides permanent.
    Stain Technol. 1953 Nov;28(6):281-3 PMID: 13113454
  13. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.
    Biochim Biophys Acta. 1966 Jan 18;114(1):158-68 PMID: 4287053
  14. Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):553-60 PMID: 5248812
  15. Chromosomes, DNA sequences, and evolution in salamanders of the genus Plethodon.
    Chromosoma. 1974;48(3):239-96 PMID: 4448107
  16. Multiple gene sites for 5S and 18 plus 28S RNA on chromosomes of Glyptotendipes barbipes (Staeger).
    J Cell Biol. 1974 Jul;62(1):132-44 PMID: 4209866
  17. Variation in rDNA redundancy level and nucleolar organizer length in normal and variant lines of the Mexican axolotl.
    J Cell Sci. 1974 Jul;15(2):239-57 PMID: 4855372
  18. Location of the genes for 5S ribosomal RNA in Xenopus laevis.
    Chromosoma. 1973;42(2):191-203 PMID: 4726606
  19. Observations on centromeric heterochromatin and satellite DNA in salamanders of the genus Plethodon.
    Chromosoma. 1973;43(4):329-48 PMID: 4776469
  20. [Demonstration on lampbrush chromosomes of Pleurodeles poireti Gervais, urodele amphibia, of a structure linked with sex, identifying the sexual bivalent and labelling the chromosome W].
    C R Acad Sci Hebd Seances Acad Sci D. 1970 Jul 6;271(1):102-4 PMID: 4988325
  21. Chromosomal localization of repetitive DNA in the newt, Triturus.
    J Cell Biol. 1972 Sep;54(3):580-91 PMID: 5065184
  22. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
    Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
  23. Amplification, polytenisation, and nucleolus organisers.
    Nat New Biol. 1973 Nov 21;246(151):81-2 PMID: 4519109
  24. Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
    Biochim Biophys Acta. 1968 Aug 23;166(1):274-6 PMID: 5698061
  25. The use of iodinated RNA for gene localization.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1860-4 PMID: 4198275
  26. Chromosomal localization of mouse satellite DNA.
    Science. 1970 Jun 12;168(3937):1356-8 PMID: 5462793
  27. Ribosomal cistrons and the nucleolar organizer.
    Biochim Biophys Acta. 1966 Feb 21;114(2):296-310 PMID: 5943882
  28. Chromosomal heteromorphism and female heterogamety in the marbled newt Triturus marmoratus (Latreille, 1800).
    Experientia. 1971 Jul;27(7):819-21 PMID: 5139507
  29. The chromosomal localization of a heavy satellite DNA in the testis of Plethodon c. cinereus.
    Chromosoma. 1971;33(2):167-82 PMID: 5577858
  30. Iodination of nucleic acids in vitro.
    Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
  31. The nucleolar organizer of Plethodon cinereus cinereus (Green). I. Location of the nucleolar organizer by in situ nucleic acid hybridization.
    Chromosoma. 1973 Jul 18;42(4):415-26 PMID: 4730559
  32. Chromosomal and nuclear location of mouse satellite DNA in individual cells.
    Nature. 1970 Mar 7;225(5236):912-5 PMID: 5415126
  33. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
    J Mol Biol. 1968 Jun 28;34(3):661-80 PMID: 5760467
  34. Localisation of reiterated nucleotide sequences in Drosophila and mouse by in situ hybridisation of complementary RNA.
    Chromosoma. 1970;31(3):331-45 PMID: 5489364
  35. Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum.
    J Cell Sci. 1966 Mar;1(1):85-108 PMID: 5326124
  36. The nucleolar organizer of Plethodon cinereus cinereus (Green). II. The lampbrush nucleolar organizer.
    Chromosoma. 1973 Jul 18;42(4):427-44 PMID: 4730560
  37. Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
    Science. 1970 Nov 6;170(3958):639-41 PMID: 5470721
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00327074
Published
1975-00-00
Pages
349-69
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327074 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2