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PMID: 109736 Published · ppublish English Journal Article

Evidence that induction and suppression of mutations and recombinations by chemical mutagens in S. cerevisiae during mitosis are jointly correlated.

Molecular & general genetics : MGG ·Vol. 168 ·No. 2 ·1979-01-10 ·Pages 125-39

Fahrig R

Abstract

Mutagen-induced intergenic and interallelic recombination as well as forward mutation were studied in one and the same strain of S. cerevisiae. In nontoxic dose ranges, the induction of mutants and recombinants was parallel after treatment with ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-methyl-N'-nitro-M-nitrosoguanidine (MNNG), triethylene melamine (TEM), 4-nitroquinoline 1-oxide (4-NQO), sodium nitrite (NaNO2), and 1-fluoro-2,4-dinitrobenzene (2,4-DNFB). Acridine orange (AO) after treatment without light induced recombinants, but reduced the frequency of spontaneous mutations. In combination with TEM, AO exerted the same effect, i.e., reduced its mutagenic effect and enhanced its recombinogenic effect. 4,5,6-Trichloro-2-(2,4-dichlorophenoxy) phenol (Cl5-predioxin) induced mutants and intergenic recombinants, but specifically reduced the spontaneous frequency of interallelic recombinants. In combination with TEM, it enhanced its mutagenic and intergenic recombinogenic effects but reduced its interallelic recombinogenic effect. The main conclusions of the present study, that is 1. Essentially similar lesions can lead to different genetic consequences, and 2. Induction of mutation and recombination are jointly correlated, i.e., suppression of mutations leads to an enhancement of recombinations, while suppression of recombinations leads to an enhancement of mutations, are used to set up a speculative concept for mutation and recombination induction in the diploid yeast cell during mitosis.

MeSH Terms
4-Nitroquinoline-1-oxide/pharmacology Acridine Orange/pharmacology Chlorophenols/pharmacology Dinitrofluorobenzene/pharmacology Drug Interactions Ethyl Methanesulfonate/pharmacology Halogenated Diphenyl Ethers Methyl Methanesulfonate/pharmacology Methylnitronitrosoguanidine/pharmacology Mitosis Mutagens/pharmacology Mutation/drug effects Phenyl Ethers/pharmacology Recombination, Genetic/drug effects Saccharomyces cerevisiae/drug effects,genetics Sodium Nitrite/pharmacology Triethylenemelamine/pharmacology
Chemicals
Chlorophenols Halogenated Diphenyl Ethers Mutagens Phenyl Ethers Methylnitronitrosoguanidine predioxin 4-Nitroquinoline-1-oxide Ethyl Methanesulfonate Methyl Methanesulfonate Dinitrofluorobenzene Acridine Orange Triethylenemelamine Sodium Nitrite
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fahrig R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00431439
Published
1979-01-10
Pages
125-39
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00431439
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