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PMID: 10997781 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The distribution of somatic H1 subtypes is non-random on active vs. inactive chromatin: distribution in human fetal fibroblasts.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 8 ·No. 5 ·2000-00-00 ·Pages 405-24

Parseghian MH, Newcomb RL, Winokur ST, Hamkalo BA

Abstract

Chromatin immunoprecipitation was employed to determine whether or not the previously reported depletion of histone H1 on actively transcribed sequences was selective with respect to H1 subtypes. DNA of immunofractionated chromatin was analyzed by slot-blots for repetitive sequences and PCR for single and low-copy sequences. Based on the analysis of a diverse set of sequences, we report distinct differences in subtype distributions. Actively transcribed chromatin, as well as chromatin poised for transcription, is characterized by a relative depletion of somatic H1 subtypes 2 and 4 (H1s-2 and H1s-4),whereas facultative and constitutive heterochromatin contain all four somatic subtypes. These results support a model in which subtypes are selectively depleted upon gene expression. In turn, the data also support the possibility that the somatic subtypes have different functional roles based on their selective depletion from different classes of DNA sequences.

MeSH Terms
Actins/genetics Cell Line Chromatin/ultrastructure Cross-Linking Reagents/metabolism DNA-Binding Proteins/genetics Fibroblasts/metabolism,ultrastructure Histones/ultrastructure Humans Immunoglobulins/genetics Lymphoid Enhancer-Binding Factor 1 Models, Genetic Nerve Tissue Proteins/genetics Plasmids/metabolism Polymerase Chain Reaction Precipitin Tests Protein-Tyrosine Kinases Proto-Oncogene Proteins c-myc/genetics Pseudogenes Receptor, Fibroblast Growth Factor, Type 3 Receptors, Fibroblast Growth Factor/genetics Tandem Repeat Sequences Transcription Factors/genetics Transcription, Genetic
Chemicals
Actins Chromatin Cross-Linking Reagents DNA-Binding Proteins Histones IgK Immunoglobulins Lymphoid Enhancer-Binding Factor 1 Nerve Tissue Proteins Proto-Oncogene Proteins c-myc Receptors, Fibroblast Growth Factor Transcription Factors postsynaptic density proteins FGFR3 protein, human Protein-Tyrosine Kinases Receptor, Fibroblast Growth Factor, Type 3
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parseghian M H
Research and Development, Techniclone Corporation, Tustin, CA 92780, USA.
Newcomb R L
Winokur S T
Hamkalo B A
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Article Info
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Abbr.
Chromosome Res
ISSN
0967-3849
DOI
10.1023/a:1009262819961
Published
2000-00-00
Pages
405-24
Language
English
Region
Netherlands
NLM ID
9313452
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1009262819961 View journal details
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