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PMID: 1102525 Published · ppublish English Journal Article

Plasmid incompatibility and control of replication: copy mutants of the R-factor R1 in Escherichia coli K-12.

Journal of bacteriology ·Vol. 124 ·No. 2 ·1975-11-00 ·Pages 641-9

Uhlin BE, Nordström K

Abstract

Plasmid incompatibility was studied in Escherichia coli K-12. By double-antibiotic selection, clones were constructed that carried the two R-factors R1 and R100, both belonging to the compatibility group FII. After release of the selection pressure, each of the two plasmids was lost at the same rate (8% per generation). Mutants of R-factor R1 showing an increased number of copies per chromosome (copy mutants) were tested for their incompatibility towards R-factor R100. The results indicate that plasmid incompatibility is quantitative and not just a qualitative property. All copy mutants studied affected incompatibility, and there were two classes of mutants: one increasing and one decreasing the incompatiblity exerted towards the test plasmid R100. Evidence is presented that incompatibility is related to the mechanisms that control replication. The implications of such a relation on proposed models for control of replication are discussed. The data do not support the hypothesis that plasmid incompatibility is due to competition for a replicational or segregational site.

MeSH Terms
Ampicillin/pharmacology Anti-Bacterial Agents/pharmacology Conjugation, Genetic DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/drug effects,enzymology,metabolism Kanamycin/pharmacology Mutation Penicillin Resistance Penicillinase/biosynthesis R Factors
Chemicals
Anti-Bacterial Agents DNA, Bacterial Kanamycin Ampicillin Penicillinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Uhlin B E
Nordström K
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37 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.2.641-649.1975
Published
1975-11-00
Pages
641-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235950
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.2.641-649.1975 PMC Full Text View journal details
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