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PMID: 4457755 Published · ppublish English Journal Article

Studies on plasmid replication. IV. Complementation of replication-defective mutants by an incompatibility-deficient plasmid.

Molecular & general genetics : MGG ·Vol. 135 ·No. 2 ·1974-00-00 ·Pages 149-61

Wyman L, Novick RP

Abstract

This paper described a complementation test system for replication-defective S. aureus pencillinase plasmids in which the incompatibility barrier has been overcome by the isolation of an incompatibility-defective (Inc-) plasmid. This plasmid appears to be stably and irreversibly integrated into the host chromosome as attempts to restore it to its original independent state have been unsuccessful. The Inc- plasmid was able to complement the thermosensitive replication defects of Seg- plasmids belonging to the same original incompatibility class but was unable to complement onels belonging to a different incompatibility class. Positive and negative phenotypic complementation tests were confirmed at the molecular level by isotopec labeling of plasmid-specific DNA molecules.

MeSH Terms
DNA Replication DNA, Bacterial Extrachromosomal Inheritance Genetics, Microbial Mutation Penicillinase/pharmacology Phenotype Staphylococcus/drug effects,growth & development
Chemicals
DNA, Bacterial Penicillinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wyman L
Novick R P
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00264782
Published
1974-00-00
Pages
149-61
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00264782
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