Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.

Molecular & general genetics : MGG ·Vol. 135 ·No. 2 ·1974-00-00 ·Pages 131-47

Novick RP

Abstract

Some 85 Staphylococcus aureus mutants phenotypically thermosensitive for penicillinase plasmid segregation (Seg-) have been isolated and characterized. Some of the mutations were plasmid-linked and those studied in detail were found to be defective in plasmid replication, most problbly at the initiation stage. Analysis of the segregation behavior of these mutants suggested a figure of 2.7 for the average number of plasmid copies per cell in a random culture. Other mutations were host chromosome-linked and these could be divided into at least three classes on the basis of their ability to maintain plasmids of the two different incompatibility sets: some were defective for type I plasmids, some for type II, and some for both types. One host mutant, defective in segregation of type I but not type II plasmids, was defective in polymerization of both.

MeSH Terms

Chromosomes DNA Replication DNA, Bacterial Extrachromosomal Inheritance Genetics, Microbial Mutation Penicillinase/pharmacology Phenotype Staphylococcus/growth & development Temperature

Chemicals

DNA, Bacterial Penicillinase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Novick R P

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26 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00264781

Published

1974-00-00

Pages

131-47

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00264781

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Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.

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