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PMID: 11027339 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 21 ·2000-10-10 ·Pages 11383-90

Gerton JL, DeRisi J, Shroff R, Lichten M, Brown PO, Petes TD

Abstract

In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by double-strand DNA breaks (DSBs). Meiotic DSBs occur at relatively high frequencies in some genomic regions (hotspots) and relatively low frequencies in others (coldspots). We used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes. Hotspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles. Coldspots were nonrandomly associated with the centromeres and telomeres.

MeSH Terms
Base Sequence Chromosome Mapping DNA Damage DNA Primers Meiosis/genetics Nucleic Acid Hybridization Recombination, Genetic Saccharomyces cerevisiae/genetics
Chemicals
DNA Primers
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gerton J L
Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
DeRisi J
Shroff R
Lichten M
Brown P O
Petes T D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.97.21.11383
Published
2000-10-10
Pages
11383-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC17209
Subset
IM
Grants
NCI NIH HHS · T32 CA009156 · United States
NHGRI NIH HHS · HG00983 · United States
NIGMS NIH HHS · R01 GM024110 · United States
NIGMS NIH HHS · GM24110 · United States
NCI NIH HHS · T32 CA09156 · United States
External Links
PubMed source DOI: 10.1073/pnas.97.21.11383 PMC Full Text View journal details
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