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PMID: 8939820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes.

Chromosoma ·Vol. 105 ·No. 5 ·1996-12-00 ·Pages 276-84

Klein S, Zenvirth D, Dror V, Barton AB, Kaback DB, Simchen G

Abstract

The preferred positions for meiotic double-strand breakage were mapped on Saccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulsed-field gel electrophoresis per 25 kb of DNA, but each chromosome had a unique distribution of DSB sites. There were no preferred meiotic DSB sites near the telomeres. DSB-prone regions were associated with all of the known "hot spots" for meiotic recombination on chromosomes I, III and VI.

MeSH Terms
Chromosomes, Artificial, Yeast/genetics Chromosomes, Fungal/genetics DNA, Recombinant Humans Meiosis/genetics Polymorphism, Genetic Recombination, Genetic/genetics Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Telomere
Chemicals
DNA, Recombinant
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Klein S
Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Zenvirth D
Dror V
Barton A B
Kaback D B
Simchen G
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF02524645
Published
1996-12-00
Pages
276-84
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02524645 View journal details
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