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PMID: 8514151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.

Genetics ·Vol. 134 ·No. 1 ·1993-05-00 ·Pages 81-150

Riles L, Dutchik JE, Baktha A, McCauley BK, Thayer EC, Leckie MP, Braden VV, Depke JE, Olson MV

Abstract

Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae are presented. In order of increasing size, they are chromosomes I, VI, III, IX, V and VIII, comprising 2.49 megabase pairs of DNA. The maps are based on the analysis of an overlapping set of lambda and cosmid clones. Overlaps between adjacent clones were recognized by shared restriction fragments produced by the combined action of EcoRI and HindIII. The average spacing between mapped cleavage sites is 2.6 kb. Five of the six chromosomes were mapped from end to end without discontinuities; a single internal gap remains in the map of chromosome IX. The reported maps span an estimated 97% of the DNA on the six chromosomes; nearly all the missing segments are telomeric. The maps are fully cross-correlated with the previously published SfiI/NotI map of the yeast genome by A. J. Link and M. V. Olson. They have also been cross-correlated with the yeast genetic map at 51 loci.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Cosmids DNA Probes DNA, Fungal/genetics Genes, Fungal Genetic Markers Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae/genetics
Chemicals
DNA Probes DNA, Fungal Genetic Markers
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Riles L
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
Dutchik J E
Baktha A
McCauley B K
Thayer E C
Leckie M P
Braden V V
Depke J E
Olson M V
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/134.1.81
Published
1993-05-00
Pages
81-150
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205446
Subset
IM
Grants
NHGRI NIH HHS · HG00334 · United States
NHGRI NIH HHS · HG00369 · United States
External Links
PubMed source DOI: 10.1093/genetics/134.1.81 PMC Full Text View journal details
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