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PMID: 3537702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Saccharomyces cerevisiae chromosome III left telomere has a type X, but not a type Y', ARS region.

Molecular and cellular biology ·Vol. 6 ·No. 4 ·1986-04-00 ·Pages 1352-6

Button LL, Astell CR

Abstract

A yeast Saccharomyces cerevisiae telomeric region was isolated by chromosome walking from HML alpha, the most distal known gene on the chromosome III left (IIIL) end. The terminal heterodisperse 3.3-kilobase (kb) SalI fragment on chromosome IIIL, 8.6 kb distal to HML alpha, was cloned in a circular vector to generate a telomeric probe. Southern hybridization and DNA sequencing analyses indicated that 0.6 kb (+/- 200 base pairs) of 5'-C1-3A-3' simple tandem repeat sequence, adjacent to a 1.2-kb type X ARS region, constitutes the telomere on the chromosome IIIL end, and no type Y' ARS region homologies exist between HML alpha and the IIIL terminus.

MeSH Terms
Base Sequence Chromosome Mapping Chromosomes/ultrastructure Cloning, Molecular DNA, Fungal/genetics Nucleic Acid Hybridization Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Button L L
Astell C R
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.4.1352-1356.1986
Published
1986-04-00
Pages
1352-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367652
Subset
IM
Databases
GENBANK
M12922
External Links
PubMed source DOI: 10.1128/mcb.6.4.1352-1356.1986 PMC Full Text View journal details
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