Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

Experientia ·Vol. 31 ·No. 10 ·1975-10-15 ·Pages 1147-9

Horisberger M, Rosset J, Bauer H

Abstract

A rapid method has been developed to visualize cell surface receptors in the SEM. Thus mannan at the surface of Candida utilis cells was localized by stabilized colloidal gold granules coated with either anti-mannan antibodies or Con A.

MeSH Terms

Antibodies, Fungal Binding Sites, Antibody Candida/immunology,ultrastructure Cell Membrane/immunology,ultrastructure Colloids Concanavalin A Gold Mannans/immunology Microscopy, Electron, Scanning/methods Saccharomyces cerevisiae/immunology,ultrastructure

Chemicals

Antibodies, Fungal Colloids Mannans Concanavalin A Gold

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Horisberger M

Rosset J

Bauer H

References (6)

6 references, click to expand

Latex spheres as markers for studies of cell surface receptors by scanning electron microscopy.
Nature. 1974 May 3;249(452):81-2 PMID: 4857323
Mannan as a major component of the bud scars of Saccharomyces cerevisiae.
Arch Mikrobiol. 1972;85(3):202-8 PMID: 4562154
An immunocolloid method for the electron microscope.
Immunochemistry. 1971 Nov;8(11):1081-3 PMID: 4110101
Scanning immunoelectron microscopy of mouse B and T lymphocytes.
Nature. 1974 Nov 8;252(5479):173-5 PMID: 4138645
Immunosorbent for the isolation of specific antibodies against mannan: localization of antigens in yeast cell walls.
Infect Immun. 1973 Mar;7(3):487-92 PMID: 4123394
Visualization of concanavalin A-binding sites with scanning electron microscopy.
J Cell Biol. 1974 Nov;63(2 Pt 1):699-707 PMID: 4417686

Article Info

Journal

Experientia

Abbr.

Experientia

ISSN

0014-4754

DOI

10.1007/BF02326761

Published

1975-10-15

Pages

1147-9

Language

English

Region

Switzerland

NLM ID

0376547

Subset

External Links

PubMed source DOI: 10.1007/BF02326761

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Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

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