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PMID: 11080146 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins.

The EMBO journal ·Vol. 19 ·No. 22 ·2000-11-15 ·Pages 5989-99

Hoiczyk E, Roggenkamp A, Reichenbecher M, Lupas A, Heesemann J

Abstract

The non-fimbrial adhesins, YadA of enteropathogenic Yersinia species, and UspA1 and UspA2 of Moraxella catarrhalis, are established pathogenicity factors. In electron micrographs, both surface proteins appear as distinct 'lollipop'-shaped structures forming a novel type of surface projection on the outer membranes. These structures, amino acid sequence analysis of these molecules and yadA gene manipulation suggest a tripartite organization: an N-terminal oval head domain is followed by a putative coiled-coil rod and terminated by a C-terminal membrane anchor domain. In YadA, the head domain is involved in autoagglutination and binding to host cells and collagen. Analysis of the coiled-coil segment of YadA revealed unusual pentadecad repeats with a periodicity of 3.75, which differs significantly from the 3.5 periodicity found in the Moraxella UspAs and other canonical coiled coils. These findings predict that the surface projections are formed by oligomers containing right- (Yersinia) or left-handed (Moraxella) coiled coils. Strikingly, sequence comparison revealed that related proteins are found in many proteobacteria, both human pathogenic and environmental species, suggesting a common role in adaptation to specific ecological niches.

MeSH Terms
Adhesins, Bacterial/chemistry,genetics,ultrastructure Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry,genetics,ultrastructure Cell Membrane/chemistry,ultrastructure Genes, Bacterial Humans Microscopy, Electron Molecular Sequence Data Moraxella catarrhalis/chemistry,genetics,ultrastructure Mutation Protein Structure, Tertiary Sequence Homology, Amino Acid Virulence Yersinia enterocolitica/chemistry,genetics,ultrastructure
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins UspA protein, Moraxella catarrhalis YadA protein, Yersinia
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hoiczyk E
Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Pettenkoferstrabetae 9a, D-80336 München, Germany.
Roggenkamp A
Reichenbecher M
Lupas A
Heesemann J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.22.5989
Published
2000-11-15
Pages
5989-99
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305836
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.22.5989 PMC Full Text View journal details
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